Genome assembly of nematode-tolerant cotton, B713 (Gossypium hirsutum L.)

The Upland cotton germplasm line BARBREN-713 (Reg. No. GP-987, PI 671965) was developed and released by the USDA-ARS, Mississippi Agricultural and Forestry Experiment Station, Texas A&M AgriLife Research and Cotton Incorporated in 2012 (Bell et al., 2015). The line significantly (LSD 0.05) reduced reproduction of root-knot and reniform nematodes in greenhouse and field assays. It also gave significant yield increases in reniform-infested fields and significantly increased yield of susceptible varieties planted in the year following BARBREN-713. Resistance to reniform nematode was transferred from Gossypium barbadense L. Acc. No. GB713 (PI 608139) and is associated primarily with the RenGB713 gene on chromosome 21. Resistance to root-knot nematode was transferred from the germplasm line M-315 RNR (PI 592514), LA 887, or Acala Nem-X and is associated primarily with the Mi-1 gene on chromosome 11. The codominant simple sequence repeat markers BNL 3279_105 and CIR 316_202 were closely linked to RenGB713 and Mi-1, respectively. A single nucleotide polymorphism marker GI-187401 also was developed to detect the Ren2GB713 gene. BARBREN-713 had fiber quality similar to M-315 RNR but yielded more than M-315 RNR in reniform-infested fields.