Ag Data Commons
Browse

File(s) stored somewhere else

Please note: Linked content is NOT stored on Ag Data Commons and we can't guarantee its availability, quality, security or accept any liability.

Generation of a rainbow trout reference transcriptome for identifying genes responding to stressors of aquaculture environments

dataset
posted on 2024-01-27, 00:24 authored by USDA Agriculture Research Service
Stressors inherent in aquaculture production can induce physiological responses which can have adverse effects on growth, reproduction and immunocompetence. We developed a reference transcriptome to evaluate responses to heat shock, low temperature, high salinity, crowding, and low dissolved oxygen/high carbon dioxide. The reference sequence was obtained by Roche 454 pyrosequencing of a normalized cDNA library and is therefore solely qualitative, containing representative samples from all the different stressors (including controls) and tissues (gill, brain, liver, spleen, kidney and muscle). A total of 3,160,306 high quality reads were generated and 2,708,437 reads were assembled into 110,026 contigs. Comparisons with existing transcriptome data reveal a significant contribution to publicly available transcriptome data for rainbow trout. This resource will be used in future studies for global characterization stress responses at the level of the transcriptome.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2011-12-20

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA80097

Usage metrics

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC