Fluorescence emission images of wood stained with acridine orange

Colonization of wood blocks, Picea glauca, by brown and white rot fungi, Postia placenta, Phanerochaete chrysosporium, and Serpula lacrymans, rapidly resulted in detectable wood oxidation, as shown by a reduced phloroglucinol response, a loss of autofluorescence, and acridine orange (AO) staining. The data included in this archive were used to refine a method for detecting changes in the chemical properties of wood. This method involves staining the sample with acridine orange and then observing the light emission using a fluorescence microscope. Data include fluorescence emission microscopic images of AO-stained wood, transmission microscopic images of phloroglucinol-stained wood, isothermal titration calorimetry results, and fluorescence emission spectra.
We used the data to develop a new method for following the course of fungal colonization of wood. In addition we stained isolated wood components in an effort to better understand the origins of the color changes in emission.
Original metadata date was 10/06/2016. Minor metadata updates on 12/16/2016.