Expression survey of Rpp1 soybean line PI200492 resistant to P. pachyrhizi

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posted on 2024-11-23, 21:26 authored by Frederick, FDWSRU, USDA

Affymetrix soybean genome arrays were used to identify genes differentially expressed in the immune resistance response at 6, 12, 24, and 48 hours after inoculation with Phakopsora pachyrhizi isolates TW72-1 or HW94-1 soybean + HW94-1 = resistant; soybean + TW72-1 = susceptible Keywords: time course Overall design: For each of 4 time points (6, 12, 24 and 48 hours post inoculation) a soybean PI200492 plant was inoculated with 2 treatments: HW94-1, or TW72-1. A mock inoculation was performed and sampled at time 0. There were three biological replicates of the entire experiment, for a total of 27 samples. During data analysis two samples failed the quality control metrics and were removed from the analysis, leaving 25 samples in the final data set.

History

Data contact name

BioProject Curation Staff

Data contact email

bioprojecthelp@ncbi.nlm.nih.gov

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2008-01-31

Theme

Non-geospatial

ISO Topic Category

biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

Public Access Level

Public

Accession Number

PRJNA101495

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA101495 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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Keywords

Glycine max eEukaryotes

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