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Examination of Colonic Hypoxia in the UC Davis Type 2 Diabetes Rat Model

dataset
posted on 2024-09-29, 07:20 authored by Arkansas Children's Nutrition Center
Type 2 diabetes is known to disrupt the composition of the gut microbiota; however, the exact mechanism that underlies this association has not been characterized. Colonocyte metabolism and its effect on luminal oxygen availability has recently been proposed as a mechanism by which the host can regulate commensal populations. Thus, our aim was to determine whether the progression of diabetes influences colonocyte oxygen levels in the UC Davis Type 2 Diabetes Mellitus (UCD-T2DM) Rat, which spontaneously develops diabetes under normal chow feeding conditions. Age-matched male UCDT2M Rats (173.8 +/- 3.6 days) prior to the onset of diabetes (PD, n=15), within 1-month post onset (RD, n=12), and 3-month post onset (D3M, n=12) were included in this study. Rats were given an IP injection of pimonidazole (PMZ, 60mg/kg body weight) 1-hour prior to euthanization. Colon tissue was fixed in 10% formalin, embedded in paraffin, and processed for immunohistochemistry detection of PMZ. Colon content microbiome was measured by 16S rRNA amplicon sequencing and content short chain fatty acids were measured by liquid chromatography-mass spectrometry.

Funding

USDA: 60626-51000-012-06S

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2023-06-15

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA984264

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA984264 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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