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Evaluating nanopore sequencing for microbial community characterization in catfish pond water

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posted on 2024-01-27, 05:13 authored by USDA ARS
The aim of this study was to identify appropriate methods for bacterial community profiling of catfish pond water with nanopore sequencing of the 16S rRNA gene. Two forward primers, two reverse primers, and three different polymerase chain reaction (PCR) cycle protocols were tested on a mock community consisting of aquaculture-relevant bacteria. After identifying the most appropriate methods, the accepted protocol was applied to water samples obtained from three catfish ponds each sampled in May, June, August, and September 2020.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2022-08-10

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA868201

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