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Euphorbia esula Gene-space sequencing and assembly

dataset
posted on 2024-01-26, 23:15 authored by USDA-ARS
Leafy spurge has been used as a model organism for studying dormancy in perennial herbaceous plants and other weediness traits. The goals of this project were to create a genomic resource for leafy spurge and to assemble the gene-space as best we could from our limited sequences. SAMN07691944 contains two different PE libraries from a collection of shoot tips from one cloned individual SAMN07691961 contains two different PE libraries from a collection of shoot tips from a (potentially) different cloned individual SAMN07693206 contains PE reads from a set of 12 different ChIP-seq libraries that showed very poor enrichment for the target sequence but which therefore contained a lot of genomic sequence data. These libraries were made from bud samples from an uncharacterized mix of individuals from a small population of plants that had been maintained clonally for many years. These samples were cleaned and processed and then used in two different assembly protocols- one in which the gene-space sequences were selected prior to assembly (PRJNA413193) and the other assembly was from the total set of cleaned fragments for whole genome assembly (PRJNA411945).

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2018-02-05

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

genomics; sequence analysis; genome

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA413193

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