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Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge

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posted on 2024-02-16, 22:12 authored by John Lippolis

Since 1964, the University of Minnesota has selectively bred a Holstein herd to maintain genetically static, unselected Holsteins (UH). Comparison of these UH cows with contemporary Holstein (CH) has demonstrated that the UH cows not only produce less milk but also have fewer health concerns than their CH herdmates. The objective of this study was to determine the effects of Holstein genotype on innate immune response in an experimental intramammary E. coli challenge model. Primiparous UH (n= 5) and CH (n = 7) cows received 430 cfu E. coli strain P4 in 1 quarter. Blood samples were collected at 0, 0.5, 1, 2, 7d relative to E. coli infusion. RNA Sequencing was conducted using the HiSeq 2500 platform (Illumina, San Diego, CA) to generate an average of 46.96 ± 2.67 million 100 bp paired end reads per sample.

For more information about this dataset contact: John Lippolis: john.lippolis@usda.gov Hao Ma: hao.ma@usda.gov


Resources in this dataset:

  • Resource Title: Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge.

    File Name: Supplemental Table 1.csv

    Resource Description: The results of RNA sequencing analysis. The raw sequencing data were submitted to NCBI SRA database with reference PRJNA808034.

Funding

USDA-ARS: 5030-32000-238-000-D

History

Data contact name

Lippolis, John

Data contact email

john.lippolis@usda.gov

Publisher

Ag Data Commons

Intended use

The effects of Holstein genotype on innate immune response.

Use limitations

Only blood sample was collected and analyzed.

Temporal Extent Start Date

2018-09-01

Temporal Extent End Date

2018-09-30

Frequency

  • continual

Theme

  • Not specified

Geographic Coverage

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Geographic location - description

Iowa, Minnesota

ISO Topic Category

  • farming
  • health

Ag Data Commons Group

  • AgBioData

National Agricultural Library Thesaurus terms

Holstein; genotype; immune response; Escherichia coli; Minnesota; herds; cows; milk; innate immunity; models; blood sampling; sequence analysis; RNA; nucleotide sequences; Breeding and Genetic Improvement; Biological Sciences

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 103

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Lippolis, John (2022). Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1524725