Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge
Since 1964, the University of Minnesota has selectively bred a Holstein herd to maintain genetically static, unselected Holsteins (UH). Comparison of these UH cows with contemporary Holstein (CH) has demonstrated that the UH cows not only produce less milk but also have fewer health concerns than their CH herdmates. The objective of this study was to determine the effects of Holstein genotype on innate immune response in an experimental intramammary E. coli challenge model. Primiparous UH (n= 5) and CH (n = 7) cows received 430 cfu E. coli strain P4 in 1 quarter. Blood samples were collected at 0, 0.5, 1, 2, 7d relative to E. coli infusion. RNA Sequencing was conducted using the HiSeq 2500 platform (Illumina, San Diego, CA) to generate an average of 46.96 ± 2.67 million 100 bp paired end reads per sample.
For more information about this dataset contact: John Lippolis: john.lippolis@usda.gov Hao Ma: hao.ma@usda.gov
Resources in this dataset:
Resource Title: Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge.
File Name: Supplemental Table 1.csv
Resource Description: The results of RNA sequencing analysis. The raw sequencing data were submitted to NCBI SRA database with reference PRJNA808034.
Funding
USDA-ARS: 5030-32000-238-000-D
History
Data contact name
Lippolis, JohnData contact email
john.lippolis@usda.govPublisher
Ag Data CommonsIntended use
The effects of Holstein genotype on innate immune response.Use limitations
Only blood sample was collected and analyzed.Temporal Extent Start Date
2018-09-01Temporal Extent End Date
2018-09-30Frequency
- continual
Theme
- Not specified
Geographic Coverage
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Iowa, MinnesotaISO Topic Category
- farming
- health
Ag Data Commons Group
- AgBioData
National Agricultural Library Thesaurus terms
Holstein; genotype; immune response; Escherichia coli; Minnesota; herds; cows; milk; innate immunity; models; blood sampling; sequence analysis; RNA; nucleotide sequences; Breeding and Genetic Improvement; Biological SciencesOMB Bureau Code
- 005:18 - Agricultural Research Service
OMB Program Code
- 005:040 - National Research
ARS National Program Number
- 103
Primary article PubAg Handle
Pending citation
- No
Public Access Level
- Public