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Diversity and selective forces in the key honey bee pathogen, Deformed wing virus

dataset
posted on 2024-01-26, 23:17 authored by USDA-ARS
Deformed wing virus (DWV) is an important pathogen of honey bees tied to widespread colony losses. The introduction of the honey bee parasitic mite, Varroa destructor, has impacted both the virulence and the diversity of DWV. Here we use high-throughput RNA sequencing to survey the genetic structure and, for the first time, test the selective forces faced by virulent DWV populations from the mainland United States. To do this, we produced a series of infectious cDNA clones corresponding to major full-length RNA genome variants (GenBank Accession numbers - MG831200 - MG831204) present in a typical virulent Varroa-associated US DWV population. Injections of these clones allowed us to assess infectivity, genetic stability of the clone-derived DWV variants, and interaction between the clones in the infected honey bees.

Funding

National Institute of Food and Agriculture, 2017-06481

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2018-01-26

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA431793

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