Data from: Wolbachia infection modifies phloem feeding behavior but not plant virus transmission by a hemipteran host
Wolbachia-infected and uninfected subpopulations of beet leafhoppers, Circulifer tenellus (Baker) (Hemiptera: Cicadellidae), co-occur in the Columbia Basin region of Washington and Oregon. While facultative endosymbionts such as Hamiltonella defensa have demonstrably altered feeding/probing behavior in hemipteran hosts, the behavioral phenotypes conferred by Wolbachia to its insect hosts, including feeding/probing, are largely understudied. We studied the feeding/probing behavior of beet leafhoppers from in-house colonies with and without Wolbachia on plants using electropenetrography, along with corresponding inoculation rates of beet curly top virus (BCTV), a phloem-limited plant pathogen vectored by beet leafhoppers. Insects carrying BCTV with and without Wolbachia were individually recorded for four hours while interacting with a potato plant, and wavelengths annotated following established conventions. Virus inoculation rates and the duration of phloem salivation events did not vary. Wolbachia-infected insects more than tripled the duration of phloem ingestion, but despite this, Wolbachia infection was linked with marginally lower, not enhanced, acquisition. Regardless, results suggest potential for Wolbachia to increase the acquisition rate of other phloem-limited plant pathogens.
Funding
USDA-ARS: 2092-22000-022-00D
History
Data contact name
Angelella, Gina, M.Data contact email
gina.angelella@usda.govPublisher
Ag Data CommonsIntended use
Data may be used in future research or meta-analysis.Temporal Extent Start Date
2023-02-01Temporal Extent End Date
2024-10-01Theme
- Non-geospatial
Geographic location - description
Wapato, Washington, USISO Topic Category
- farming
- biota
National Agricultural Library Thesaurus terms
Wolbachia; phloem; feeding behavior; virus transmission; Circulifer tenellus; basins; Washington (state); Oregon; endosymbionts; hosts; phenotype; insects; Beet curly top virus; plant pathogens; Solanum tuberosum; potatoes; wavelengths; viruses; salivation; ingestionOMB Bureau Code
- 005:18 - Agricultural Research Service
OMB Program Code
- 005:040 - National Research
ARS National Program Number
- 304
Pending citation
- Yes
Related material without URL
Angelella, G.M, J.J. Foutz, and J. Galindo-Schuller. 2024. Wolbachia infection modifies phloem feeding behavior but not plant virus transmission by a hemipteran host. Journal of Insect Physiology: 104746.Public Access Level
- Public