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Data from: Transcriptomes of bovine ovarian follicular and luteal cells

posted on 2024-02-09, 18:10 authored by Sarah M. Romereim, Adam F. Summers, William E. Pohlmeier, Pan Zhang, Xiaoying Hou, Heather Talbott, Robert A. Cushman, Jennifer R. Wood, John S. Davis, Andrea S. Cupp

Affymetrix Bovine GeneChip® Gene 1.0 ST Array RNA expression analysis was performed on four somatic ovarian cell types: the granulosa cells (GCs) and theca cells (TCs) of the dominant follicle and the large luteal cells (LLCs) and small luteal cells (SLCs) of the corpus luteum. The normalized linear microarray data was deposited to the NCBI GEO repository (GSE83524). Subsequent ANOVA determined genes that were enriched (≥2 fold more) or decreased (≤−2 fold less) in one cell type compared to all three other cell types, and these analyzed and filtered datasets are presented as tables. Genes that were shared in enriched expression in both follicular cell types (GCs and TCs) or in both luteal cells types (LLCs and SLCs) are also reported in tables. The standard deviation of the analyzed array data in relation to the log of the expression values is shown as a figure. These data have been further analyzed and interpreted in the companion article "Gene expression profiling of ovarian follicular and luteal cells provides insight into cellular identities and functions", Romereim et al., (2017) Mol. Cell. Endocrinol. 439:379-394.

Resources in this dataset:

  • Resource Title: RNA Expression Data from Four Isolated Bovine Ovarian Somatic Cell Types.

    File Name: Web Page, url:

    NCBI Gene Expression Omnibus (GEO) Accession Display. Analysis of the RNA present in each bovine cell type using Affymetrix microarrays yielded new cell-specific genetic markers, functional insight into the behavior of each cell type via Gene Ontology Annotations and Ingenuity Pathway Analysis, and evidence of small and large luteal cell lineages using Principle Component Analysis. Enriched expression of select genes for each cell type was validated by qPCR. This expression analysis offers insight into the lineage and differentiation process that transforms somatic follicular cells into luteal cells. The orignal Affymetrix .CEL files and the normalized linear expression data are included in this submission.


USDA: NEB 26-202/W2112


USDA: NEB 26-206

USDA-NIFA: 2013-67015-20965

USDA: 2016-67012-24697

USDA: 2014-67011-22280


Data contact name

Cupp, Andrea S.

Data contact email


Data in Brief

Intended use

The follicular GC and TC transcriptome data can be compared to previously published bovine gene expression analyses for corroboration and are valuable in metadata analyses investigating GC and TC transcriptomes at different stages or in different species. There are no previously published transcriptomes available for large luteal cells (LLC) and small luteal cells (SLC). Therefore, the luteal cell gene expression data allow novel insight into these two cell types. Lists of identified genes that are specifically enriched in each somatic ovarian cell type can inform future physiological research on the functions of the ovarian somatic cells.


  • Not specified

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

animal ovaries; cattle; genes; RNA; granulosa cells; luteal cells; microarray technology; analysis of variance; data collection; statistical analysis; gene expression regulation; follicular atresia; transcriptome; cows; gene expression; messenger RNA; corpus luteum

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Romereim, S. M., Summers, A. F., Pohlmeier, W. E., Zhang, P., Hou, X., Talbott, H. A., Cushman, R. A., Wood, J. S., Davis, J. S., & Cupp, A. S. (2017). Transcriptomes of bovine ovarian follicular and luteal cells. Data in Brief 10: 335-339.