posted on 2024-02-13, 13:44authored bySurendra Neupane, Febina M. Mathew, Adam J. Varenhorst, Madhav P. Nepal
<p>Soybean aphid (<em>Aphis glycines</em>; SBA) and soybean cyst nematode (<em>Heterodera glycines</em>; SCN) are two major pests of soybean (<em>Glycine max</em>) in the United States of America. This study aims to characterize three-way interactions among soybean, SBA, and SCN using both demographic and genetic datasets. SCN-resistant and SCN-susceptible soybean cultivars with a combination of soybean aphids (biotype 1) and SCN (HG type 0) in a randomized complete block design (RCBD) with six blocks were used to evaluate the three-way interactions in a greenhouse setup. Treatments receiving SCN were infested at planting with 2000 nematode eggs, and the treatments with soybean aphids were infested at second trifoliate growth stage (V2) with 15 soybean aphids. The whole roots were sampled from plants at 5 and 30 days post SBA infestation for RNA sequencing using Illumina Hiseq. 3000. The data comprises of 47 libraries that are useful for further analyses of important genes, which are involved in interaction effects of SBA and SCN on soybean. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Data records: Transcriptome profiling of interaction effects of soybean cyst nematodes and soybean aphids on soybean.</p> <p>File Name: Web Page, url: <a href="https://www.nature.com/articles/s41597-019-0140-4#Sec7">https://www.nature.com/articles/s41597-019-0140-4#Sec7</a> </p></li></ul><p></p>
The 47 RNA libraries will be useful for analyses of important genes involved in interaction effects of soybean aphid and soybean cyst nematode on soybean plants.
Neupane, Surendra; Mathew, Febina M.; Varenhorst, Adam J.; Nepal, Madhav P. (2019). Data from: Transcriptome profiling of interaction effects of soybean cyst nematodes and soybean aphids on soybean. Scientific Data. https://doi.org/10.1038/s41597-019-0140-4