Data from: Toll-like receptor ligands enhance vaccine efficacy against virulent Newcastle disease virus challenge in chickens
To enhance the efficacy of the current Newcastle disease vaccine, we have tested two potential adjuvants (Imiquimod and ODN-1826) in chickens. Birds were treated with PBS, Imiquimod or ODN-1826 (50 µg/bird) or vaccinated intranasally with live LaSota strain with or without Imiquimod or ODN-1826 (50 µg/bird). Two weeks after vaccination, birds were challenged with virulent New-castle disease virus (chicken/CA/212676/2002). The experimental setting was a laboratory with animal care as approved by the institutional animal care and use committee as appropriate for the species and age of bird. Data are the serum antibody titers to the vaccine or challenge virus as determined by hemagglutination inhibition assay. Virus shedding titers by the oral and cloacal route for individual birds exposed to virulent Newcastle disease virus were determined by quantitative real-time RT-PCR. Expression of antiviral genes from tissues collected at 1 and 3 days after treating 2 week old SPF chickens with adjuvant and/or vaccines were determined by quantitative PCR.
Resources in this dataset:
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Hemagglutination inhibition (HI) antibody titers 14 days after vaccinating 2-week-old SPF chickens with LaSota vaccine with or without adjuvant (ODN-1826 or Imiquimod) File Name: Lee et al., Serology Data I from Adjuvanted live NDV vaccine study.csv
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Hemagglutination inhibition antibody titers 14 days after infecting 4 week old SPF chickens with virulent Newcastle disease virus (California/212676/2002) File Name: Lee et al., Serology Data II from Adjuvanted live NDV vaccine study.csv
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Cycle threshold (Ct) value of viral RNA from swabs collected at 2 and 4 days after infecting 4-week-old SPF chickens with virulent Newcastle disease virus (California/212676/2002) File Name: Lee et al.,Virus Shedding Data from Adjuvanted live NDV vaccine study.csv
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Cycle threshold (Ct) value of target specific mRNA collected at 1 and 3 days after treating 2-week-old SPF chickens with adjuvant and/or vaccines File Name: Lee et al.,Transcription Data from Adjuvanted live NDV vaccine study.csv
Funding
USDA-ARS: 6040-32000-082-00D
USDA-ARS: ARSX2020
History
Data contact name
Lee, ChangData contact email
chang.lee@usda.govPublisher
Ag Data CommonsIntended use
A full description of the methods is presented in the associated publication. May be useful for meta-analyses of related studies in evaluating vaccine efficacy and immune response.Use limitations
Group sizes (n=3) for transcription data is small.Temporal Extent Start Date
2022-11-01Temporal Extent End Date
2023-09-08Frequency
- notPlanned
Theme
- Not specified
Geographic Coverage
{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-83.358053863049,33.928106192905]},"type":"Feature","properties":{}}]}Geographic location - description
934 College Station Rd., Athens, GeorgiaISO Topic Category
- environment
- farming
National Agricultural Library Thesaurus terms
Toll-like receptors; ligands; vaccines; Avian orthoavulavirus 1; chickens; Newcastle disease; adjuvants; vaccination; virulence; viruses; animal care; Institutional Animal Care and Use Committee; blood serum; antibodies; hemagglutination inhibition test; viral shedding; cloaca; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; genes; meta-analysis; antibody formationOMB Bureau Code
- 005:18 - Agricultural Research Service
OMB Program Code
- 005:040 - National Research
ARS National Program Number
- 103
Pending citation
- No
Public Access Level
- Public
