Ag Data Commons

sorry, we can't preview this file

journal.pone_.0167566.s001.csv (4.64 kB)

Data from: The Majority of Genotypes of the Virulence Gene inlA Are Intact among Natural Watershed Isolates of Listeria monocytogenes from the Central California Coast

Download (4.64 kB)
posted on 2024-02-13, 13:12 authored by Lisa Gorski, Craig T. Parker, Anita S. Liang, Samarpita Walker, Kelly F. Romanolo

Internalin A is an essential virulence gene involved in the uptake of the foodborne pathogen Listeria monocytogenes into host cells. It is intact in clinical strains and often truncated due to Premature Stop Codons (PMSCs) in isolates from processed foods and processing facilities. Less information is known about environmental isolates. We sequenced the inlA alleles and did Multi Locus Variable Number Tandem Repeat Analysis (MLVA) on 112 L. monocytogenes isolates from a 3-year period from naturally contaminated watersheds near a leafy green growing area in Central California. The collection contained 14 serotype 1/2a, 12 serotype 1/2b, and 86 serotype 4b strains. Twenty-seven different inlA alleles were found. Twenty-three of the alleles are predicted to encode intact copies of InlA, while three contain PMSCs. Another allele has a 9-nucleotide deletion, previously described for a clinical strain, indicating that it is still functional. Intact inlA genes were found in 101 isolates, and 8 isolates contained the allele predicted to contain the 3-amino acid deletion. Both allele types were found throughout the 3-year sampling period. Three strains contained inlA alleles with PMSCs, and these were found only during the first 3 months of the study. SNP analysis of the intact alleles indicated clustering of alleles based on serotype and lineage with serotypes 1/2b and 4b (lineage I strains) clustering together, and serotype 1/2a (lineage II strains) clustering separately. The combination of serotype, MLVA types, and inlA allele types indicate that the 112 isolates reflect at least 49 different strains of L. monocytogenes. The finding that 90% of environmental L. monocytogenes isolates contain intact inlA alleles varies significantly from isolates found in processing plants. This information is important to public health labs and growers as to the varieties of L. monocytogenes that could potentially contaminate fresh produce in the field by various means.

Resources in this dataset:

  • Resource Title: S1 Table. Isolate information and inlA genotype sorted by allele type.

    File Name: journal.pone_.0167566.s001.csv

    Resource Description: Alleles labeled “1 + letter” were isolated more than once, and are predicted to encode intact 800 amino acid proteins. Alleles labeled with “RM + number” indicate unique alleles only isolated once. Allele 2 are identical to each other and are predicted to encode a functional 797 aa protein. Allele 3 contain PMSCs , and 3A-1 and 3A-2 are predicted to encode heterologous 262 aa proteins, and 3B is predicted to encode a 576 aa protein.

    Taken from the online resource located at


USDA-ARS: 5325-42000-050-00D

U.S. Food and Drug Administration: 224-11-2044


Data contact name

Gorski, Lisa

Data contact email



Temporal Extent Start Date


Temporal Extent End Date



  • Not specified

Geographic Coverage


Geographic location - description

Central California coast

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

virulence; food pathogens; Listeria monocytogenes; stop codon; processed foods; alleles; loci; tandem repeat sequences; multiple-locus variable number tandem-repeat analysis; watersheds; green leafy vegetables; California; serotypes; sequence deletion; single nucleotide polymorphism; public health; growers; fresh produce; genotype; coasts

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Gorski, Lisa; Parker, Craig T.; Liang, Anita S.; Walker, Samarpita; Romanolo, Kelly F. (2018). Data from: The Majority of Genotypes of the Virulence Gene inlA Are Intact among Natural Watershed Isolates of Listeria monocytogenes from the Central California Coast. PLOS One.

Usage metrics



    Ref. manager