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Data from: Tephritid fruit fly gut bacterial population and community dynamics following adult emergence

dataset
posted on 2023-11-30, 12:15 authored by Charles MasonCharles Mason

Data include microbial count data (CFUs), 16S-rRNA copy number data (qPCR), and microbial community (microbiome) data from the guts of the invasive tephritid fruit flies, melon fly (Zeugodacus cucurbitae) and medfly (Ceratitis capitata).

Resources in this dataset:

  • Resource Title: R code for dada2 processing and stacked bar charts of control microbiomes File Name: Control_Processing.zip Resource Description: Data showing performance of known controls (purchased from Zymo Research) using in-house DNA extraction and PCR methods for 16S-rRNA gene amplification and sequencing.

  • Resource Title: Data processing of 16S amplicon data File Name: 16S SSU rRNA Microbiome Data Processing and Analysis.zip Resource Description: Raw data and accompanying R scripts for analysis of figure and generation of figures and tables. Data files include both amplicon sequence variant (ASV) count data matrix and accompanying ASV sequence files and taxonomies. Analysis and figure generation are made through independent R files.

  • Resource Title: Data and analysis of fly culturable titers File Name: CFU titers.zip Resource Description: Colony forming units (CFUs) of fruit flies at different ages and the R code for figure generation and analysis.

  • Resource Title: qPCR of 16S rRNA of Tephritid fruit flies at different ages File Name: 16S qPCR Titers.zip Resource Description: Raw data and R code of 16S rRNA copy numbers associated with medfly and melon fly gut tissues.

Funding

Agricultural Research Service, 2040-22430-028-000-D

History

Data contact name

Mason, Charles

Data contact email

charles.mason@usda.gov

Publisher

Ag Data Commons

Temporal Extent Start Date

2021-10-01

Temporal Extent End Date

2021-12-18

Theme

  • Not specified

Geographic Coverage

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ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

fruit flies; digestive system; microbial communities; microbiome; invasive species; Zeugodacus cucurbitae; Ceratitis capitata; gene amplification; ribosomal RNA; genes; metagenomics

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 304

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Mason, Charles J. (2023). Data from: Tephritid fruit fly gut bacterial population and community dynamics following adult emergence. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1529269