posted on 2023-11-30, 09:36authored byManan SharmaManan Sharma, Pushpinder K. Litt, Bryan T. Vinyard, Kali E. Kniel
<p><em>Escherichia coli</em> survival in soils containing either composted poultry litter (CPL), heat-treated poultry pellets (HTPP), poultry litter (PL) or unamended (chemical fertilizer). Test plots were either covered with plastic mulch (M) or not mulched (NoM). The study was conducted in 2018 and 2019 during cucumber growing seasons at the University of Delaware research farm and each study lasted 120 days. Data from the current study were collected to examine the survival of non-pathogenic <em>Escherichia coli</em> and transfer to cucumbers grown in same field in two separate years. Soil moisture, total nitrogen, nitrate, total carbon, soluble carbon, soluble solids, rainfall, soil temperature and air temperature, along with the number of days needed for <em>E. coli</em> to decline by 4 log CFU/gdw, were included in random forest models used to a) predict 4-log declines of <em>E. coli</em> inoculated to soils and b) transfer of <em>E. coli</em> to cucumbers from soils with different biological soil amendments.
The data included here are specifically for other investigators to use to make different forms or versions of three different statistical models used in the submitted manuscript. Data for three models are included:
1) Dpi4log, the number of days needed for <em>E. coli</em> levels in various combinations of year, amendment and mulch, were calculated by applying sigmoidal (single, double, triple, or quadruple) model to <em>E. coli</em> data collected over time.
2) A random forest model using soil and weather data was used to determine which factors listed above best predicted dpi4log values. This model accounted for 98% of the observed variance.
3) A random forest model using soil and weather data, along with dpi4log, was used to predict transfer of <em>E. coli</em> to soils from cucumbers (log MPN/cucumber). This model accounted for approximately 63% of the variance in the study. </p>
<div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Graph of E. coli levels over 120 days in soils under various conditions.</p> <p>File Name: Graphs of Fitted Sigmoidal Regression Models onto Observed gEclog vs DPI.pdf</p><p>Resource Description: Graph of *E. coli* levels in 24 different combinations of year, amendment, and mulch status over 120 days </p></li><br><li><p>Resource Title: Comparison of actual model-generated log CFU/gdw data .</p> <p>File Name: Observed and Sigmoidal Model Predicted gEcLog values - Daily Increment.csv</p><p>Resource Description: Comparison of sigmoidal model-generated log CFU/gdw vs observed data </p></li><br><li><p>Resource Title: Soil temperature, Air temperature and Cumulative Rainfall observed in 2018 and 2019.</p> <p>File Name: Soil air temp cumulative rainfall 2018 2019.xlsx</p><p>Resource Description: These are the climate data used to inform and predict E. coli survival in soils containing biological and chemical fertilizer</p></li><br><li><p>Resource Title: Data set used in Random Forest model to predict transfer of E. coli from soils to cucumber fruits .</p> <p>File Name: UD ARS Cucumber Study Consolidated Data Version 2 Single Transference Column Original Data Scale.csv</p><p>Resource Description: This data set includes the sigmoidal model-estimated values of dpi4log (the number of days needed to achieve 4 log decline in E. coli levels) in this model </p></li><br><li><p>Resource Title: Dataset used in Random Forest model to identify variables and factors which predict dpi4log values of E. coli in soils containing biological soil amendment of animal origin.</p> <p>File Name: Formatted Soil Data for Random Forest Analysis.xlsx</p><p>Resource Description: Dataset used in the Random Forest model to identify variables and factors which predict dpi4log values - the number of days needed to observe a 4 log reduction, estimated by sigmoidal modeling of collected E. coli data - of E. coli in soils containing biological soil amendment of animal origin</p></li></ul><p></p>
Funding
U.S. Food and Drug Administration, IAA-224-17-2023S
Agricultural Research Service, Project Plan 8042-32420-006-00-D
The intended use of this data set is for investigators interested in examining the survival of enteric pathogens in soils amended with treated or untreated biological soil amendments of animal origin (BSAAO) and their potential transfer to growing fruits and vegetables. The survival of enteric pathogens in soils and their potential transfer to produce is an area that requires more data to populate robust statistical models to conduct the risk assessment needed to determine to the appropriate time interval between BSAAO application and harvest of fruits and vegetables.
Use limitations
The limitation of these data are that they can solely be used in reference to the E. coli measured in soil plots. Soil and weather parameters measured and recorded may be used to use the affect of rainfall on the soil parameters measured in relation to E. coli survival in BSAAO-amended soils.
Escherichia coli; poultry manure; heat treatment; poultry; pellets; mineral fertilizers; plastic film mulches; mulching; cucumbers; growing season; Delaware; farms; soil water; total nitrogen; nitrates; carbon; total soluble solids; rain; soil temperature; air temperature; soil amendments; statistical models; data collection; algorithms; meteorological data; variance; food safety; manure amendments
OMB Bureau Code
005:18 - Agricultural Research Service
OMB Program Code
005:040 - National Research
ARS National Program Number
108
Pending citation
No
Public Access Level
Public
Preferred dataset citation
Sharma, Manan; Litt, Pushpinder K.; Vinyard, Bryan T.; Kniel, Kali E. (2020). Data from: Temporal and agricultural factors influence E. coli survival in soil and transfer to cucumbers. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1520517