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Data from: Modulation of the Gut Microbiota Structure and Function by Two Structurally Different Lemon Pectins

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posted on 2023-12-19, 15:26 authored by Jenni Firrman, Karley K. Mahalak, Jamshed Bobokalonov, LinShu Liu

In vitro study of the effects on the human gut microbiota of lemon pectins with two different molecular weights and having varying degrees of esterification. Data collected following incubations include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (found in the NCBI Sequence Read Archive associated with BioProject PRJNA903836: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA903836), RT-qPCR of Bifidobaterium sp. 16S rRNA genes, and short-chain fatty acid concentrations.

Resources in this dataset:

  • Resource Title: Short Chain Fatty Acid concentrations File Name: pectin_SCFA_data.csv

  • Resource Title: Pectin_total_bacterial_qPCR_data File Name: pectin_total_bacterial_qPCR_data.csv

  • Resource Title: Pectin_bifidobacterium_genus_qPCR_data File Name: pectin_bifidobacterium_genus_qPCR_data.csv

  • Resource Title: Sample metadata

  • File Name: pectin_sample_attributes.csv

Funding

USDA-ARS: 8072-41000-108-000D

History

Data contact name

Narrowe, Adrienne

Data contact email

adrienne.narrowe@usda.gov

Publisher

Ag Data Commons

Temporal Extent Start Date

2018-10-16

Theme

  • Not specified

Geographic Coverage

{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-75.187092456102,40.078126249741]},"type":"Feature","properties":{}}]}

Geographic location - description

Eastern Regional Research Center, Wyndmoor, PA, USA

ISO Topic Category

  • health

National Agricultural Library Thesaurus terms

intestinal microorganisms; pectins; in vitro studies; humans; lemons; sequence analysis; ribosomal RNA; genes; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; short chain fatty acids; fatty acid composition; nucleotide sequences

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 306

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Firrman, Jenni; Mahalak, Karley K.; Bobokalonov, Jamshed; Liu, LinShu (2023). Data from: Modulation of the Gut Microbiota Structure and Function by Two Structurally Different Lemon Pectins. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1529137

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    Categories

    Keywords

    16S rRNA sequence datashort chain fatty acidspectinRT-qPCRNP306data.govARS

    Licence

    CC BY 4.0

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