posted on 2024-02-15, 22:47authored byTaylor Van Winkle, Marco A. Ponce, Hannah E. Quellhorst, Alexander Bruce, Chloe E. Albin, Tania N. Kim, Kun Yan Zhu, William MorrisonWilliam Morrison
<p>This dataset is associated with the forthcoming publication entitled, "Microbial volatile organic compounds mediate attraction by a primary but not secondary stored product insect pest in wheat", and includes data on grain damage from near infrared spectroscopy, behavioral data from wind tunnel and release-recapture experiments, as well as volatile characterization of headspace from moldy grain. For all files, incubation intervals 9, 18, and 27 d represent how long grain was incubated after being tempered to a grain moisture of 12, 15, or 19% or left untempered (ctrl; 10.8% grain moisture). TSO = Trece storgard oil; empty = negative control (no stimulus), LGB = lesser grain borer (<em>Rhzyopertha dominica</em>), and RFB = red flour beetle (<em>Tribolium castaneum</em>).</p>
<p>Note: The resource 'GC/MS Grain MVOC Headspace Data' was added 2021-08-04 with the deletion of some compounds as unlikely natural compounds and potential contaminants. This is the dataset that undergirds the non-metric multidimensional scaling analysis.</p>
<p>See the included file list for more information about methods and results of each file in this dataset. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: GC-MS/Headspace Data.</p> <p>File Name: tvw_final_gc_ms_data.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Microbial damage on wheat evaluated with near-infrared spectroscopy.</p> <p>File Name: tvw_nearinfrared_sorting_damaged_grain_fungal_exp.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Release-Recapture Datasets with LGB & RFB.</p> <p>File Name: tvw_rr_lgb_rfb_microbial_cues.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: Wind tunnel response by RGB & LGB.</p> <p>File Name: tvw_wt_lgb_rfb_data_microbial_cues.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: GC/MS Grain MVOC Headspace Data.</p> <p>File Name: taylor_headspace_final_data_peer_reviewed_ag_commons.csv</p><p>Resource Software Recommended: Microsoft Excel,url: <a href="https://www.microsoft.com/en-us/microsoft-365/excel">https://www.microsoft.com/en-us/microsoft-365/excel</a> </p></li><br><li><p>Resource Title: README file list.</p> <p>File Name: file_list_MVOCwheat.txt</p></li></ul><p></p>
Funding
USDA-NIFA: 2017-70006-27262
USDA-ARS: ARS Innovation Funds Account#992-0142-901 Sub 3
The goal of the study was to evaluate the behavioral response of T. castaneum and R. dominica to the MVOCs produced by stored grain. We were also interested in the effects of grain moisture (12, 15, and 19% grain moisture) and incubation period 9, 18, or 27 d) on insect behavioral responses. We characterized the volatile emissions from each of these treatments, and tentatively identified key compounds in each of the headspaces from the grain and microbial activity. We hypothesized that MVOCs may be more important for the secondary feeder, T. castaneum, because MVOCs may signal that otherwise unusable intact grains have become susceptible by weakening of the kernel through fungal activity. This may allow the secondary pest to more easily penetrate the bran of a kernel. Further, we hypothesize that higher grain moisture and incubation periods will have greater fungal activity.
Van Winkle, Taylor; Ponce, Marco A.; Quellhorst, Hannah E.; Bruce, Alexander; Albin, Chloe E.; Kim, Tania N.; Zhu, Kun Yan; Morrison, William R. (2021). Data from: Microbial volatile organic compounds mediate attraction by a primary but not secondary stored product insect pest in wheat. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1520888