Data from: Microbial volatile organic compounds mediate attraction by a primary but not secondary stored product insect pest in wheat
This dataset is associated with the forthcoming publication entitled, "Microbial volatile organic compounds mediate attraction by a primary but not secondary stored product insect pest in wheat", and includes data on grain damage from near infrared spectroscopy, behavioral data from wind tunnel and release-recapture experiments, as well as volatile characterization of headspace from moldy grain. For all files, incubation intervals 9, 18, and 27 d represent how long grain was incubated after being tempered to a grain moisture of 12, 15, or 19% or left untempered (ctrl; 10.8% grain moisture). TSO = Trece storgard oil; empty = negative control (no stimulus), LGB = lesser grain borer (Rhzyopertha dominica), and RFB = red flour beetle (Tribolium castaneum).
Note: The resource 'GC/MS Grain MVOC Headspace Data' was added 2021-08-04 with the deletion of some compounds as unlikely natural compounds and potential contaminants. This is the dataset that undergirds the non-metric multidimensional scaling analysis.
See the included file list for more information about methods and results of each file in this dataset.
Resources in this dataset:
Resource Title: GC-MS/Headspace Data.
File Name: tvw_final_gc_ms_data.csv
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel
Resource Title: Microbial damage on wheat evaluated with near-infrared spectroscopy.
File Name: tvw_nearinfrared_sorting_damaged_grain_fungal_exp.csv
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel
Resource Title: Release-Recapture Datasets with LGB & RFB.
File Name: tvw_rr_lgb_rfb_microbial_cues.csv
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel
Resource Title: Wind tunnel response by RGB & LGB.
File Name: tvw_wt_lgb_rfb_data_microbial_cues.csv
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel
Resource Title: GC/MS Grain MVOC Headspace Data.
File Name: taylor_headspace_final_data_peer_reviewed_ag_commons.csv
Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel
Resource Title: README file list.
File Name: file_list_MVOCwheat.txt
Funding
USDA-NIFA: 2017-70006-27262
USDA-ARS: ARS Innovation Funds Account#992-0142-901 Sub 3
USDA-ARS: 3020-43000-033-00D
History
Data contact name
Morrison, William R.Data contact email
william.morrison@usda.govPublisher
Ag Data CommonsIntended use
The goal of the study was to evaluate the behavioral response of T. castaneum and R. dominica to the MVOCs produced by stored grain. We were also interested in the effects of grain moisture (12, 15, and 19% grain moisture) and incubation period 9, 18, or 27 d) on insect behavioral responses. We characterized the volatile emissions from each of these treatments, and tentatively identified key compounds in each of the headspaces from the grain and microbial activity. We hypothesized that MVOCs may be more important for the secondary feeder, T. castaneum, because MVOCs may signal that otherwise unusable intact grains have become susceptible by weakening of the kernel through fungal activity. This may allow the secondary pest to more easily penetrate the bran of a kernel. Further, we hypothesize that higher grain moisture and incubation periods will have greater fungal activity.Temporal Extent Start Date
2018-06-11Temporal Extent End Date
2020-12-31Theme
- Not specified
Geographic Coverage
{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-96.599122881917,39.195671210229]},"type":"Feature","properties":{}}]}ISO Topic Category
- biota
National Agricultural Library Thesaurus terms
volatile organic compounds; storage insects; wheat; data collection; near-infrared spectroscopy; wind tunnels; headspace analysis; molds (fungi); oils; Rhyzopertha dominica; Tribolium castaneum; chemical ecology; entomology; trapping; stored grain; emissions; microbial activity; branOMB Bureau Code
- 005:18 - Agricultural Research Service
OMB Program Code
- 005:040 - National Research
ARS National Program Number
- 304
Primary article PubAg Handle
Pending citation
- No
Public Access Level
- Public