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Data from: Life history metrics for Culex tarsalis (Diptera: Culicidae) and Culicoides sonorensis (Diptera: Ceratopogonidae) are not impacted by artificial feeding on defibrinated versus EDTA-treated blood

posted on 2024-02-01, 22:02 authored by Dustin Swanson, Bethany McgregorBethany Mcgregor

Artificial blood feeding is a common practice for the study and maintenance of blood-feeding arthropod colonies. Commercially purchased blood is often treated to prevent clot-formation using either mechanical or chemical means. For many hematophagous insects, the effect that different anticoagulation methods may have on life history metrics is unclear. In the current study, Culex tarsalis Coquillett and Culicoides sonorensis Wirth & Jones were fed blood treated with either mechanical (defibrination) or chemical (K2 EDTA) anticoagulation methods. Several blood feeding and life history metrics were evaluated between treatment groups including proportion blood feeding, fecundity, fertility, and mortality. No significant differences were found for any of the measured life history metrics for either species. For experiments measuring aspects of these blood feeding and life history traits, blood treated using either defibrination or K2 EDTA anticoagulants should not impact experimental outcomes. These studies were conducted using laboratory reared colony populations of Culex tarsalis and Culicoides sonorensis. The effect of these anticoagulation methods on life history metrics of field collected populations could be different.


USDA-ARS: 3020-32000-018-00D


Data contact name

McGregor, Bethany L.

Data contact email


Ag Data Commons

Intended use

These data may be used to evaluate the biological impact of using K2 EDTA treated or defibrinated blood products on proportion feeding, fertility, fecundity, and mortality in laboratory reared colony Culex tarsalis or Culicoides sonorensis insects.

Use limitations

The effect of these anticoagulation methods on life history metrics of field collected populations or laboratory reared populations that are managed using different methods could be different. For these populations, additional studies should be conducted to determine how anticoagulation methods might impact biological traits.

Temporal Extent Start Date


ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

life history; Culex tarsalis; Culicoides sonorensis; nutritional support; blood; hematophagy; hematophagous insects; EDTA (chelating agent); fecundity; mortality; anticoagulants; laboratory rearing

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 104

ARIS Log Number


Pending citation

  • No

Public Access Level

  • Public

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