posted on 2024-09-23, 21:09authored byHannah E. Quellhorst, Maria Sakka, Georgia V. Baliota, William MorrisonWilliam Morrison, Christos G. Athanassiou
<p dir="ltr"><i>Insects</i></p><p dir="ltr">Adult <i>P. truncatus </i>were obtained from insect colonies kept in the Laboratory of Entomology and Agricultural Zoology (LEAZ), at the Department of Agriculture, Crop Production and Rural Environment, University of Thessaly, Greece, on whole maize kernels, at 26°C and 55% relative humidity (RH) and continuous darkness.</p><p dir="ltr"><i>Insecticide-Incorporated Netting</i></p><p dir="ltr">The experiments were carried out in plastic petri dishes 90 mm in diameter (50.4 cm<sup>2 </sup>bottom surface). The inside of each petri dish was covered with two types of LLIN (0.4% deltamethrin, D-Terrence, Vestergaard INC., Lausanne Switzerland; and 0.34% alpha-cypermethrin, Carifend, BASF Ag, Ludwigshafen, Germany) and a polytetraflurorethylene preparation (Fluon, 60 wt% dispersion in water, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) to prevent insects from escaping. An additional series of dishes with physically identical control netting were prepared without an insecticide treatment to serve as the control. Twenty <i>P. truncatus</i> were then exposed on the insecticide-treated netting in petri dishes for 60, 90, 120, 240 min, 1, 3, and 5 days.</p><p dir="ltr"><i>Mortality and Recovery</i></p><p dir="ltr">After exposure, insects were evaluated for mortality and individual <i>P. truncatus</i> that remained alive or knocked down (not dead) were then place into clean Petri dish arenas with a small amount of clean cracked maize kernels and evaluated for delayed mortality after 7 days. Using a stereomicroscope (SMZ-18, Nikon Inc., Tokyo, Japan) under 60× magnification, <i>P. truncatus</i> were classified as alive (moving normally, is able to right itself when flipped over, no twitching), affected (moving sluggishly or erratically, unable to right itself, twitching of antennae or legs may be present), or dead (completely immobile even after prodding) according to prior published definitions. There were three 3 replicates for each time treatment and netting type and 3 subreplicates for a total of 9 replicates for each treatment combination.</p><p dir="ltr"><i>Explanation of files</i></p><p dir="ltr">The file "Greece Net Data 2023_All" includes the raw mortality ratings, whereas "Greece Net Data 2023_Recovery" includes calculated recovery values by P. truncatus, where recovery was calculated to "alive" from the initial reading (0 d) in "Greece Net Data 2023_All". </p>
Funding
Maximizing Adoption by Demonstrating the Compatibility of Insecticide Netting with Diverse Pest Management Tactics at Food Facilities
Only for examining mortality or larger grain borer.
Use limitations
Only as above.
Temporal Extent Start Date
2023-09-01
Temporal Extent End Date
2024-11-30
Frequency
daily
Theme
Non-geospatial
Geographic location - description
University of Thessaly, Department of Agriculture, Plant Production and Rural Development, Laboratory of Entomology and Agricultural Zoology
Phytokou str., 38443, N. Ionia, Volos, Greece
Hannah Quellhorst, Maria Sakka, Georgia Baliota, William. R. Morrison III, and Christos G. Athanassiou. Laboratory evaluation of two insecticide-incorporated nets for the management of Prostephanus truncatus. Journal of Economic Entomology, in review.