<p dir="ltr">We conducted a fly rearing experiment to characterize diet-induced changes in the microbiome of female <i>Bactrocera dorsalis</i>. In order to explicitly investigate the impacts of larval diet on the microbiome, including potential stable bacterial constituents of <i>B. dorsalis</i>, we performed 16S rRNA sequencing on the gut tissues of teneral female flies reared from 4 different host fruits (guava, mango, papaya, and rose-apple) infested using a single cohort of wild <i>B. dorsalis</i> that emerged from tropical almond (mother flies). We provide processed data and R code for our analysis.</p><p dir="ltr">Raw data (.fastq files) for analysis are located at NCBI SRA under accession number PRJNA1061202</p>