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Data from: Heritable differences in abundance of bacterial rhizosphere taxa are correlated with fungal necrotrophic pathogen resistance

dataset
posted on 2024-02-14, 05:59 authored by Cloe S. Pogoda, Kyle G. Keepers, Stephan Reinert, Zahirul I. Talukder, Brian SmartBrian Smart, Jason A. Corwin, Kennedy L. Money, Erin C.E. Collier-Zans, William Underwood, Thomas J. Gulya, Alisha Quandt, Nolan C. Kane, Brent S. Hulke

This Ag Data Commons submission includes the 94 sunflower paired-end sequencing FASTQ files, the corresponding 16S bacterial FASTQ files, and other relevant data to the study described below:

Host-microbe interactions are increasingly recognized as important drivers of organismal health, growth, longevity, and community-scale ecological processes. However, less is known about how genetic variation affects hosts' associated microbiomes and downstream phenotypes. We demonstrate that sunflower Helianthus annuus harbors substantial, heritable variation in microbial communities under field conditions. We show that microbial communities co-vary with heritable variation in resistance to root infection caused by the necrotrophic pathogen Sclerotinia sclerotiorum, and that plants grown in autoclaved soil showed almost complete elimination of pathogen resistance. Association mapping suggests at least 59 genetic locations with effects on both microbial relative abundance and Sclerotinia resistance. Although the genetic architecture appears quantitative, we have elucidated previously unexplained genetic variation for resistance to this pathogen. We identify new targets for plant breeding and demonstrate the potential for heritable microbial associations to play important roles in defense in natural and human-altered environments.

See README for details of each table in the spreadsheet and related information.

Funding

USDA-ARS: 58-3060-8-019

History

Data contact name

Hulke, Brent

Data contact email

brent.hulke@usda.gov

Publisher

Ag Data Commons

Intended use

This is the sunflower sequence data (paired-end FASTQ) and corresponding 16S bacterial and ITS fungal data related to the paper titled "Heritable differences in abundance of bacterial rhizosphere taxa are correlated with fungal necrotrophic pathogen resistance".

Temporal Extent Start Date

2017-08-01

Theme

  • Not specified

Geographic Coverage

{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-99.121611,47.507595]},"type":"Feature","properties":{}}]}

Geographic location - description

Carrington, ND; Greenhouse in Fargo, ND

ISO Topic Category

  • biota
  • farming

National Agricultural Library Thesaurus terms

rhizosphere; fungi; pathogens; Helianthus annuus; host-pathogen relationships; longevity; genetic variation; hosts; microbiome; phenotype; microbial communities; disease resistance; root diseases; Sclerotinia sclerotiorum; autoclaving; soil; chromosome mapping; plant breeding; internal transcribed spacers; bacteria; bioinformatics

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 301

Pending citation

  • No

Related material without URL

Cloe S. Pogoda, Kyle G. Keepers, Stephan Reinert, Zahirul I. Talukder, Brian C. Smart, Ziv Attia, Jason A. Corwin, Kennedy L. Money, Erin C.E. Collier-zans, William Underwood, Thomas J. Gulya, C. Alisha Quandt, Nolan C. Kane, Brent S. Hulke (in review). Heritable differences in abundance of bacterial rhizosphere taxa are correlated with fungal necrotrophic pathogen resistance. Molecular Ecology.

Public Access Level

  • Public

Preferred dataset citation

Pogoda, Cloe S.; Keepers, Kyle G.; Reinert, Stephan; Talukder, Zahirul I.; Smart, Brian C.; Corwin, Jason A.; Money, Kennedy L.; Collier-Zans, Erin C.E.; Underwood, William; Gulya, Thomas J.; Quandt, Alisha; Kane, Nolan C.; Hulke, Brent S. (2023). Data from: Heritable differences in abundance of bacterial rhizosphere taxa are correlated with fungal necrotrophic pathogen resistance. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1529803