Ag Data Commons
Browse

File(s) stored somewhere else

Please note: Linked content is NOT stored on Ag Data Commons and we can't guarantee its availability, quality, security or accept any liability.

Data from: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks

dataset
posted on 2024-02-15, 19:49 authored by Kylie BendeleKylie Bendele, Felix D. Guerrero, Deanna M. Bodine

This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.

The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts.


Resources in this dataset:

Funding

USDA-ARS: 62053200003100D

USDA-ARS: 3094-32000-036-00D

History

Data contact name

Bendele, Kylie

Data contact email

Kylie.Bendele@usda.gov

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2008-11-17

Temporal Extent End Date

2008-11-18

Theme

  • Not specified

Geographic Coverage

{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-98.32857,26.38684]},"type":"Feature","properties":{}}]}

Geographic location - description

USDA-ARS Cattle Fever Tick Research Laboratory, United States Department of Agriculture, 22675 N. Moorefield Rd, Edinburg, TX 78541, USA

ISO Topic Category

  • biota

Ag Data Commons Group

  • Veterinary Pest Genomics Center

National Agricultural Library Thesaurus terms

gene expression; adults; females; Rhipicephalus microplus; ticks; transcriptome; genome; cattle; odors; gene expression regulation; models; animals; sequence analysis; engorgement; Ixodidae; livestock and meat industry; pests; mining; transcription (genetics); tick control

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 104

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Bendele, K.G., Guerrero, Cameron C., Bodine, D.M., Miller, R.J. (2019). Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks. Experimental & Applied Acarology 79:107-124

Usage metrics

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC