Data from: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks
This study produced a comprehensive transcriptome from newly molted adult ticks and will provide a useful resource for studies of tick feeding and host perception and also assist genome annotation refinements.
The goal was to develop a comprehensive transcriptome of adult female Rhipicephalus microplus ticks and discover transcriptome changes that are related to feeding and host-perception prior to feeding. We have sequenced the transcriptomes of unfed newly molted adult female ticks, newly molted adult females that were feeding upon a bovine host for 3 hours following attachment to the host, and newly molted adult females that were placed in a gas-permeable bag attached to a bovine host for 3 hours (attachment to host prevented but host odors available to the ticks). There were 2 biological replicates and transcriptomes assembled from each individual experimental condition. A collective adult female transcriptome was assembled from sequence data pooled from all 3 conditions (using data from all replicates). Differential gene expression was examined using all possible comparisons to detect putative feeding-associated and host perception-associated transcripts.
Resources in this dataset:
Resource Title: Gene expression during the early stages of host perception and attachment in adult female Rhipicephalus microplus ticks.
File Name: Web Page, url: https://doi.org/10.1007/s10493-019-00420-1
Funding
USDA-ARS: 62053200003100D
USDA-ARS: 3094-32000-036-00D
History
Data contact name
Bendele, KylieData contact email
Kylie.Bendele@usda.govPublisher
National Center for Biotechnology InformationTemporal Extent Start Date
2008-11-17Temporal Extent End Date
2008-11-18Theme
- Not specified
Geographic Coverage
{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-98.32857,26.38684]},"type":"Feature","properties":{}}]}Geographic location - description
USDA-ARS Cattle Fever Tick Research Laboratory, United States Department of Agriculture, 22675 N. Moorefield Rd, Edinburg, TX 78541, USAISO Topic Category
- biota
Ag Data Commons Group
- Veterinary Pest Genomics Center
National Agricultural Library Thesaurus terms
gene expression; adults; females; Rhipicephalus microplus; ticks; transcriptome; genome; cattle; odors; gene expression regulation; models; animals; sequence analysis; engorgement; Ixodidae; livestock and meat industry; pests; mining; transcription (genetics); tick controlOMB Bureau Code
- 005:18 - Agricultural Research Service
OMB Program Code
- 005:040 - National Research
ARS National Program Number
- 104
Primary article PubAg Handle
Pending citation
- No
Public Access Level
- Public