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Data from: Fructooligosaccharides (FOS) differentially modifies the in vitro gut microbiota in an age-dependent manner

dataset
posted on 2024-02-15, 19:49 authored by Karley K. Mahalak, LinShu Liu

A 24-h in vitro culturing method was used to determine whether fructooligosaccharide (FOS) treatment effects on the human gut microbiome has a differential effect by age, using fecal samples from 18 adults in 3 age groups with 6 individuals for each group, young adult (25–35 years of age), adult (36–50 years of age), and older adult (51–70 years of age). Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883714: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883714), total bacterial qPCR, Bifidobacterium genus specific q-PCR, and short chain fatty acid concentrations.

Resources in this dataset:

  • Resource Title: Sample metadata File Name: FOS_sample_attributes.csv

  • Resource Title: Short Chain Fatty Acid concentrations File Name: FOS_SCFA_data.csv

  • Resource Title: Total bacterial and Bifidobacterium genus qPCR File Name: total_bacteria_and_Bifidobacterium_specific_qPCR.csv

Funding

USDA-ARS: 8072-41000-108-000D

History

Data contact name

Narrowe, Adrienne

Data contact email

adrienne.narrowe@usda.gov

Publisher

Ag Data Commons

Temporal Extent Start Date

2021-05-01

Theme

  • Not specified

Geographic Coverage

{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-75.187092456102,40.078126249741]},"type":"Feature","properties":{}}]}

Geographic location - description

Ghent, Belgium and Philadelphia, PA, USA

ISO Topic Category

  • health

National Agricultural Library Thesaurus terms

fructooligosaccharides; intestinal microorganisms; humans; ribosomal RNA; quantitative polymerase chain reaction; Bifidobacterium; short chain fatty acids; fatty acid composition; nucleotide sequences; prebiotics

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 306

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Mahalak, Karley K.; Liu, LinShu (2023). Data from: Fructooligosaccharides (FOS) differentially modifies the in vitro gut microbiota in an age-dependent manner. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1529151

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