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Data from: Estimation of genetic parameters and their sampling variances for quantitative traits in the type 2 modified augmented design

posted on 2024-02-13, 13:12 authored by Frank M. You, Qijian Song, Gaofeng Jia, Yanzhao Cheng, Scott D. Duguid, Helen M. Booker, Sylvie J. Cloutier

The type 2 modified augmented design (MAD2) is an efficient unreplicated experimental design used for evaluating large numbers of lines in plant breeding and for assessing genetic variation in a population. Statistical methods and data adjustment for soil heterogeneity have been previously described for this design. In the absence of replicated test genotypes in MAD2, their total variance cannot be partitioned into genetic and error components as required to estimate heritability and genetic correlation of quantitative traits, the two conventional genetic parameters used for breeding selection. We propose a method of estimating the error variance of unreplicated genotypes that uses replicated controls, and then of estimating the genetic parameters. Using the Delta method, we also derived formulas for estimating the sampling variances of the genetic parameters. Computer simulations indicated that the proposed method for estimating genetic parameters and their sampling variances was feasible and the reliability of the estimates was positively associated with the level of heritability of the trait. A case study of estimating the genetic parameters of three quantitative traits, iodine value, oil content, and linolenic acid content, in a biparental recombinant inbred line population of flax with 243 individuals, was conducted using our statistical models. A joint analysis of data over multiple years and sites was suggested for genetic parameter estimation. A pipeline module using SAS and Perl was developed to facilitate data analysis and appended to the previously developed MAD data analysis pipeline (

Resources in this dataset:


Agriculture and Agri-Food Canada

Genome Canada

Western Grains Research Foundation


Data contact name

You, Frank M.

Data contact email


Science Direct


  • Not specified

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

quantitative traits; experimental design; plant breeding; genetic variation; statistical analysis; soil heterogeneity; genotype; variance; heritability; genetic correlation; computer simulation; case studies; iodine value; lipid content; linolenic acid; inbred lines; flax; statistical models

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

You, Frank M.; Song, Qijian; Jia, Gaofeng; Cheng, Yanzhao; Duguid, Scott D.; Booker, Helen M.; Cloutier, Sylvie J. (2018). Data from: Estimation of genetic parameters and their sampling variances for quantitative traits in the type 2 modified augmented design. Science Direct.