posted on 2024-02-08, 19:34authored byAlexander P. Tuckow, Kevin B. Temeyer, Pia Untalan Olafson, Adalberto A. Pérez de León
<p>This dataset reports discovery and initial comparative analysis of 88 presumptive microRNA (miRNA) sequences from the stable fly, obtained using high-throughput sequencing of small RNAs. The majority of stable fly miRNAs were 22-23 nucleotides (nt) in length. Many miRNAs were arthropod specific, and several mature miRNA sequences showed greater sequence identity to miRNAs from other blood-feeding dipterans such as mosquitoes rather than to Drosophilids. This initial step in characterizing the stable fly microRNAome provides a basis for further analyses of life stage-specific and tissue-specific expression to elucidate their functional roles in stable fly biology.</p>
<p>The stable fly, Stomoxys calcitrans (L.), is a serious ectoparasite affecting animal production and health of both animals and humans. Stable fly control relies largely on chemical insecticides; however, the development of insecticide resistance as well as environmental considerations requires continued discovery research to develop novel control technologies. MicroRNAs are a class of short noncoding RNAs that have been shown to be important regulators of gene expression across a wide variety of organisms, and may provide an innovative approach with regard to development of safer more targeted control technologies. </p><div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Table 1. miRNAs identified in pooled life stages of the stable fly S. calcitrans and corresponding miRBase 18 entries.</p> <p>File Name: Web Page, url: <a href="https://academic.oup.com/view-large/91020692">https://academic.oup.com/view-large/91020692</a> </p><p>miRNAs identified in pooled life stages of the stable fly S. calcitran. and corresponding miRBase 18 entries (≥10 reads, sorted by abundance).</p></li></ul><p></p>
Knipling-Bushland U.S. Livestock Insects Research Laboratory, Kerrville, TX 780280-09184
ISO Topic Category
biota
farming
Ag Data Commons Group
Veterinary Pest Genomics Center
OMB Bureau Code
005:18 - Agricultural Research Service
OMB Program Code
005:040 - National Research
ARS National Program Number
104
Pending citation
No
Public Access Level
Public
Preferred dataset citation
Tuckow, Alexander P.; Temeyer, Kevin B.; Olafson, Pia Untalan; Pérez de León, Adalberto A. (2015). Data from: Discovery of MicroRNAs of the Stable Fly (Diptera: Muscidae) by High-Throughput Sequencing. Journal of Medical Entomology.