Data from: Discovery of MicroRNAs of the Stable Fly (Diptera: Muscidae) by High-Throughput Sequencing
This dataset reports discovery and initial comparative analysis of 88 presumptive microRNA (miRNA) sequences from the stable fly, obtained using high-throughput sequencing of small RNAs. The majority of stable fly miRNAs were 22-23 nucleotides (nt) in length. Many miRNAs were arthropod specific, and several mature miRNA sequences showed greater sequence identity to miRNAs from other blood-feeding dipterans such as mosquitoes rather than to Drosophilids. This initial step in characterizing the stable fly microRNAome provides a basis for further analyses of life stage-specific and tissue-specific expression to elucidate their functional roles in stable fly biology.
The stable fly, Stomoxys calcitrans (L.), is a serious ectoparasite affecting animal production and health of both animals and humans. Stable fly control relies largely on chemical insecticides; however, the development of insecticide resistance as well as environmental considerations requires continued discovery research to develop novel control technologies. MicroRNAs are a class of short noncoding RNAs that have been shown to be important regulators of gene expression across a wide variety of organisms, and may provide an innovative approach with regard to development of safer more targeted control technologies.
Resources in this dataset:
Resource Title: Table 1. miRNAs identified in pooled life stages of the stable fly S. calcitrans and corresponding miRBase 18 entries.
File Name: Web Page, url: https://academic.oup.com/view-large/91020692
miRNAs identified in pooled life stages of the stable fly S. calcitran. and corresponding miRBase 18 entries (≥10 reads, sorted by abundance).
Funding
USDA-ARS
History
Data contact name
Temeyer, Kevin B.Data contact email
kevin.temeyer@usda.govPublisher
Journal of Medical EntomologyTheme
- Not specified
Geographic location - description
Knipling-Bushland U.S. Livestock Insects Research Laboratory, Kerrville, TX 780280-09184ISO Topic Category
- biota
- farming
Ag Data Commons Group
- Veterinary Pest Genomics Center
OMB Bureau Code
- 005:18 - Agricultural Research Service
OMB Program Code
- 005:040 - National Research
ARS National Program Number
- 104
Pending citation
- No
Public Access Level
- Public