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Data from: Detection of viral, bacterial, and protozoan pathogens and microbial source tracking markers in paired large- and small-volume water samples

dataset
posted on 2025-05-27, 15:26 authored by JOSEPH HEFFRONJOSEPH HEFFRON, Joel Stokdyk, Aaron Firnstahl, Rachel Cook, Claire Hruby, Mark Borchardt

This repository contains data supporting the publication, "Detection of viral, bacterial, and protozoan pathogens and microbial source tracking markers in paired large- and small-volume water samples." The dataset comprises qPCR concentrations of microbial targets in paired large volume and small volume samples from field studies and laboratory recovery experiments, as described in the main publication.

"Field studies.csv" contains sample data and target concentrations for three field studies, two in groundwater and one in surface water. In the "Groundwater - Private wells (n = 138)" study, large volume samples taken by dead-end ultrafiltration were compared to small volume grab samples. In the remaining two studies, large volume dead-end ultrafiltration and small volume samples were collected synchronously to remove the effect of spatial/temporal heterogeneity during collection. Only the 15 qPCR assays with detections and common to all studies are included. Geographic information is not included to protect the privacy of study participants.

"Recovery data.csv" contains laboratory data for large volume and small volume method recovery of three microbial targets (a bacterium, a virus, and a protozoan) at varying initial concentrations.

"Storage data.csv" contains laboratory data for microbial target decay during storage prior to processing. Liquid small volume samples and large volume ultrafilters were stored at 4 C to simulate normal sample storage and transport conditions. Concentrations of three microbial targets (a bacterium, a virus, and a protozoan) were determined for storage times from 0 to 96 hours.

Descriptive data for all microbial targets is provided in "Target data.csv". An explanation of variables (with units) for all data files can found in "Key to variables.csv".

Funding

USDA-ARS: 5090-12630006-000D

History

Data contact name

Heffron, Joe

Data contact email

joseph.heffron@usda.gov

Publisher

Ag Data Commons

Temporal Extent Start Date

2019-04-09

Temporal Extent End Date

2021-10-04

Frequency

  • asNeeded

Theme

  • Non-geospatial

ISO Topic Category

  • environment

National Agricultural Library Thesaurus terms

Protozoa; pathogens; quantitative polymerase chain reaction; groundwater; surface water; wells; ultrafiltration; spatial data; bacteria; viruses; liquids; storage temperature

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 212

ARIS Log Number

426224

Pending citation

  • Yes

Related material without URL

Heffron, Joe; Stokdyk, Joel; Firnstahl, Aaron; Cook, Rachel; Hruby, Claire; Borchardt, Mark. (In Review.) Detection of viral, bacterial, and protozoan pathogens and microbial source tracking markers in paired large- and small-volume water samples.

Public Access Level

  • Public