Data from: Detection of the 1016Gly and 989Pro knockdown resistance mutations in Florida, USA Aedes aegypti

Aedes aegypti is a major arboviral disease vector and is therefore a critical target for control by public health programs. Early eradication efforts have shown that Ae. aegypti can rapidly build insecticide resistance and now, resistance to pyrethroids, the major class of adulticides used for operational control, is common in many populations. A major contributor to this resistance is a group of knockdown resistance (kdr) SNPs that normally exist in distinct ensembles in the Western Hemisphere and the Indopacific with little known mixing. In this study, we detected, and confirmed using multiple methods, two Indopacific kdr SNPs, V1016G and S989P, in three recent collections from Osceola County, Florida. This represents a large expansion of the geographic range of the Indopacific kdr SNPs. We consider the implications of this finding on future insecticide resistance surveillance studies, including assessing the ability of our current screening tools to detect these SNPs. We find that the presence of the Indopacific SNPs requires modification of existing resistance screening protocols and requires further work to understand the operational implications for mosquito control.

The information in this repository consists of the data supporting the original detection and the confirmation of the presence of the Indopacific mutations. It also includes sequencing protocols, analysis scripts, and sequencing metadata used in the study.