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Data from: Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay
A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. The dataset includes expression values for drpt, il11a, il1b1, il1b2, il1b3, il1r-like-1(e3-5), il1r-like-1(e9-11), il1r1-like-a, il1r1-like-b, il1r2, saa, tnfa1, tnfa2, tnfa3, tnfrsf1a, tnfrsf1a-like-a, tnfrsf1a-like-b, tnfrsf5, and tnfrsf9. Gene expression was measured at four time-points post-challenge in both a resistant line (ARS-Fp-R) and a susceptible line (ARS-Fp-S) of rainbow trout. In addition, fish body weight, spleen index and the Flavobacterium psychrophilum load are reported. These data are an extension of information presented and discussed in the article "Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss)" (Kutyrev et al., 2016).
Resources in this dataset:
Resource Title: Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay.
File Name: Web Page, url: https://www.sciencedirect.com/science/article/pii/S2352340917300331
Data in Brief data article providing expression values for a suite of immune-relevant genes in rainbow trout (Oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. Data are available for download as Excel spreadsheet.
Resource Software Recommended: Microsoft Excel,url: https://office.microsoft.com/excel/
Funding
USDA-ARS: 1930-32000-006
USDA-NIFA: 2012-67015-30217
History
Data contact name
Wiens, Gregory D.Data contact email
greg.wiens@ars.usda.govPublisher
Data in BriefIntended use
Data describe the optimization and validation of a new GeXP assay for measuring rainbow trout proinflammatory cytokines. The data include PCR product relative migration and identification that are of use to researchers when performing this assay. The gene expression values from the rainbow trout resistant line, ARS-Fp-R, and the susceptible line, ARS-Fp-S, can be used in future studies for meta-analyses comparing the response to other pathogens or environmental conditions.Theme
- Not specified
ISO Topic Category
- biota
National Agricultural Library Thesaurus terms
data collection; cytokine receptors; gene expression; Oncorhynchus mykiss; reverse transcriptase polymerase chain reaction; genes; tumor necrosis factors; interleukin-1; ligands; fish; body weight; spleen; Flavobacterium psychrophilum; disease resistance; bacterial cold-water disease; immunogenetics; polymerase chain reaction; meta-analysis; pathogens; environmental factors; aquaculture; genetic lines; microbial load; quantitative polymerase chain reaction; principal component analysis; gene expression regulation; immune response; selection methods; receptorsOMB Bureau Code
- 005:18 - Agricultural Research Service
OMB Program Code
- 005:040 - National Research
Primary article PubAg Handle
Pending citation
- No
Public Access Level
- Public
