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Data from: Characterization of Adult Transcriptomes from the Omnivorous Lady Beetle Coleomegilla maculata Fed Pollen or Insect Egg Diet

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posted on 2024-02-13, 13:11 authored by Margaret Allen

Expressed genes from two individual sibling specimens of Coleomegilla maculata (Coleoptera: Coccinellidae). One individual was fed only insect eggs as an adult, and one was fed only pollen as an adult. Two sequenced samples, total RNA from a single individual adult specimen of Coleomegilla maculata, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage.

BioProject: Coleomegilla maculata strain:inbred Mississippi Transcriptome or Gene expression Taxonomy: Coleomegilla maculata Project data type: Transcriptome or Gene expression Scope: Multiisolate US Department of Agriculture Accession: PRJNA236444 ID: 236444


Resources in this dataset:

  • Resource Title: Total RNA from individual adult fed only eggs as adult; first one of two files (paired Illumina)..

    File Name: filtered_LEO_TGACCA_L002_R1fastq.gz

    Resource Description: Two sequenced samples, total RNA from a single individual adult specimen of Coleomegilla maculata, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage. BioProject: Coleomegilla maculata strain:inbred Mississippi Transcriptome or Gene expression Taxonomy: Coleomegilla maculata Project data type: Transcriptome or Gene expression Scope: Multiisolate US Department of Agriculture Accession: PRJNA236444 ID: 236444 Coleomegilla maculata (Coleoptera: Coccinellidae). Common name unassigned, but commonly referred to as Pink Lady Beetle. Native to North America, widespread. Beneficial insect that feeds on spider mites, Lepidoptera eggs, eggs of Colorado potato beetle and many others.


  • Resource Title: Total RNA sequences from single adult C. maculata fed insect eggs as adult. Second of two (paired Illumina) files..

    File Name: filtered_LEO_TGACCA_L002_R2fastq.gz

    Resource Description: Two sequenced samples, total RNA from a single individual adult specimen of Coleomegilla maculata, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage. BioProject: Coleomegilla maculata strain:inbred Mississippi Transcriptome or Gene expression Taxonomy: Coleomegilla maculata Project data type: Transcriptome or Gene expression Scope: Multiisolate US Department of Agriculture Accession: PRJNA236444 ID: 236444 Coleomegilla maculata (Coleoptera: Coccinellidae). Common name unassigned, but commonly referred to as Pink Lady Beetle. Native to North America, widespread. Beneficial insect that feeds on spider mites, Lepidoptera eggs, eggs of Colorado potato beetle and many others. Sequenced by U. of Wash http://www.htseq.org/


  • Resource Title: Total RNA sequenced from one adult lady beetle fed pollen as adult. First of two (paired Illumina) files..

    File Name: filtered_PO_CGATGT_L002_R1fastq.gz

    Resource Description: Two sequenced samples, total RNA from a single individual adult specimen of Coleomegilla maculata, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage. BioProject: Coleomegilla maculata strain:inbred Mississippi Transcriptome or Gene expression Taxonomy: Coleomegilla maculata Project data type: Transcriptome or Gene expression Scope: Multiisolate US Department of Agriculture Accession: PRJNA236444 ID: 236444 Coleomegilla maculata (Coleoptera: Coccinellidae). Common name unassigned, but commonly referred to as Pink Lady Beetle. Native to North America, widespread. Beneficial insect that feeds on spider mites, Lepidoptera eggs, eggs of Colorado potato beetle and many others. Sequenced by U. of Wash http://www.htseq.org/


  • Resource Title: Total RNA from pollen-fed adult lady beetle. Second of two (paired Illumina) files..

    File Name: filtered_PO_CGATGT_L002_R2fastq.gz

    Resource Description: Two sequenced samples, total RNA from a single individual adult specimen of Coleomegilla maculata, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage. BioProject: Coleomegilla maculata strain:inbred Mississippi Transcriptome or Gene expression Taxonomy: Coleomegilla maculata Project data type: Transcriptome or Gene expression Scope: Multiisolate US Department of Agriculture Accession: PRJNA236444 ID: 236444

Funding

USDA-ARS: 6402-22000-062-00D

History

Data contact name

Allen, Margaret L.

Data contact email

meg.allen@usda.gov

Publisher

Ag Data Commons

Intended use

To understand the biology of an important beneficial insect in US agroecosystems. To identify genetic markers associated with diet and nutrition, with an aim to mass produce augmentative biological control organisms that are healthy and fit.

Theme

  • Not specified

ISO Topic Category

  • biota
  • environment
  • farming

National Agricultural Library Thesaurus terms

genomics; adults; transcriptome; omnivores; Coleomegilla maculata; pollen; diet; genes; Coccinellidae; insect eggs; RNA; agroecosystems; North America; carnivores; plant-based diet; insects; rearing; siblings; larvae; Mississippi; gene expression; taxonomy; USDA; beneficial insects; biological control; nutritional adequacy

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 304

Primary article PubAg Handle

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Allen, Margaret (2018). Data from: Characterization of Adult Transcriptomes from the Omnivorous Lady Beetle Coleomegilla maculata Fed Pollen or Insect Egg Diet. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1461648

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