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Data from: Biological control of boxwood blight by Pseudomonas protegens recovered from recycling irrigation systems

dataset

posted on 2024-02-13, 13:26 authored by Xiao Yang, Chuanxue Hong

Boxwood (Buxus spp.) blight caused by Calonectria pseudonaviculata (Cps) is an emerging fungal disease in the United States. It has caused significant loss to the ornamental nursery and landscape industries. In this study, 1547 bacterial strains recovered from recycling irrigation systems were evaluated in vitro and in planta for their biological control efficacy against Cps. Three most potent strains 13A3, 14D5, and 34B6 identified all belonged to Pseudomonas protegens. Strains 13A3 and 14D5 reduced Cps mycelial growth by 95 to 100% in two sets of in vitro experiments including a 48-well plate assay and a dual culture assay. Strain 34B6 also reduced Cps growth by 93% in the 48-well plate assay and 66 to 72% in dual culture plates. In planta, strain 14D5 reduced Cps infection by more than 50%, while strains 13D3 and 34B6 by 40 to 46%. Pseudomonas protegens strains produced antifungal secondary metabolites that reduced Cps growth and infection. Other possible modes of action against boxwood blight are discussed.

Resources in this dataset:

Resource Title: Supplementary Table S1.

File Name: Web Page, url: https://www.sciencedirect.com/science/article/pii/S1049964418300434?via=ihub#s0095
Bacterial strains, the reduction of Cps growth in the in-vitro screening and area under disease progress curve (AUDPC) in the in-planta screening, and species identities and GenBank accession numbers of 147 identified bacterial strains.

Funding

USDA-APHIS: 14-8130-0282-CA

USDA-APHIS: 15-8130-0282-CA

History

Data contact name

Yang, Xiao

Data contact email

yxiao9@vt.edu

Publisher

Biological Control

Intended use

To screen diverse bacterial strains recovered from recycling irrigation systems against Cps in dual culture, to identify promising strains to species level by DNA sequencing, and to further evaluate them using in planta assays for their potential as BCAs of boxwood blight.

Temporal Extent Start Date

2012-01-01

Temporal Extent End Date

2013-12-31

Theme

Not specified

Geographic Coverage

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Geographic location - description

Eastern Virginia

ISO Topic Category

biota
farming

National Agricultural Library Thesaurus terms

Buxus sempervirens; blight; Calonectria; fungi; United States; landscapes; industry; irrigation systems; biological control; Pseudomonas protegens; mycelium; in vitro studies; secondary metabolites; mechanism of action; Cylindrocladium; ornamental trees; fungal diseases of plants; plant pathogenic fungi; nursery crops; biopesticides; microbial pesticides; biological control agents; microbial toxins; fungal antagonists; toxigenic strains; emerging diseases

Primary article PubAg Handle

https://pubag.nal.usda.gov/catalog/6365586

Pending citation

Public Access Level

Public

Preferred dataset citation

Yang, Xiao; Hong, Chuanxue (2019). Data from: Biological control of boxwood blight by Pseudomonas protegens recovered from recycling irrigation systems. Biological Control.