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Data from: Bacterial communities and prevalence of antibiotic resistance genes carried within house flies (Diptera: Muscidae) associated with beef and dairy cattle farms

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posted on 2024-02-15, 18:01 authored by Saraswoti NeupaneSaraswoti Neupane, Justin L. Talley, David B. Taylor, Dana Nayduch

House flies (Musca domestica L.) are vectors of human and animal pathogens at livestock operations. Microbial communities in flies are acquired from, and correlate with, their local environment. However, variation among microbial communities carried by flies from farms in different geographical areas is not well understood. We characterized bacterial communities of female house flies collected from beef and dairy farms in Oklahoma, Kansas, and Nebraska and further evaluated the prevalence of antibiotic resistance genes in bacteria within flies. We evaluated the influence of farm type and farm location on bacterial communities, diversity, pathogenic bacteria strains and prevalence of antibiotic resistance genes. These data can be used for better understanding of abundance and prevalence of bacterial communities in house flies associated with livestock operations. These data were collected in September 2019. Abbreviations used include Operational Taxonomic Units(OTUs), Canonical Correspondence analysis (CCA), Infectious Bovine Keratoconjunctivitis (IBK), Anti Microbial Resistance (AMR), and Antibiotic Resistance Genes (ARGs).

The raw Illumina MiSeq sequence data for this project can be found here: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA863664

Resources in this dataset:

  • Resource title: Metadata for Microbiome of House Fly Associated with Cattle Farms File name: Metadata for Microbiome of House Fly Associated with Cattle Farms.xlsx Resource description: This spreadsheet links the raw sequence reads on NCBI with data on farm type, farm location and sample type.

Funding

USDA-ARS: 3020-32000-018-00D

History

Data contact name

Neupane, Saraswoti

Data contact email

sneupane@ksu.edu

Publisher

Ag Data Commons

Intended use

These data can be used for better understanding of abundance and prevalence of bacterial communities in house flies associated with livestock operations.

Use limitations

These data should not be used to make inferences for ecosystems other than house fly microbiome associated with livestock operations. These data were collected in September and therefore are not representative of bacterial communities present in other months.

Temporal Extent Start Date

2019-09-04

Temporal Extent End Date

2019-09-17

Theme

  • Not specified

Geographic Coverage

{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-97.093122,36.131563]},"type":"Feature","properties":{}},{"geometry":{"type":"Point","coordinates":[-97.129722,36.135556]},"type":"Feature","properties":{}},{"geometry":{"type":"Point","coordinates":[-96.589632,39.22503]},"type":"Feature","properties":{}},{"geometry":{"type":"Point","coordinates":[-96.596691,39.226537]},"type":"Feature","properties":{}},{"geometry":{"type":"Point","coordinates":[-96.544565,40.553283]},"type":"Feature","properties":{}},{"geometry":{"type":"Point","coordinates":[-96.483723,41.143362]},"type":"Feature","properties":{}}]}

ISO Topic Category

  • biota
  • farming

National Agricultural Library Thesaurus terms

bacterial communities; antibiotic resistance genes; Musca domestica; dairy farming; animal pathogens; bacteria; beef cattle; dairy cattle; Moraxella bovoculi; Moraxella bovis; tetracycline; florfenicol

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 104

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Neupane, Saraswoti; Talley, Justin L.; Taylor, David B.; Nayduch, Dana (2023). Data from: Bacterial communities and prevalence of antibiotic resistance genes carried within house flies (Diptera: Muscidae) associated with beef and dairy cattle farms. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1529546