Data from: Association mapping of resistance to tan spot in the Global Durum Panel

Seedlings of the GDP were also infiltrated with cultures containing the necrotrophic effectors (NEs) Ptr ToxA and Ptr ToxB. Approximately 20 μl of NE-containing culture filtrate (CF) was infiltrated into two plants (1 leaf per plant) in each cone using a needleless 1-ml syringe. The infiltrated area (~3 cm) was marked with permanent marker on the leaves, and the plants were placed in a growth chamber (21 °C, 12-h photoperiod) for 5 days. The reactions were scored using a 0–3 scale where 0 indicated no reaction and 3 indicated severe necrosis or chlorosis. The expansion of chlorosis beyond the boundaries of Ptr ToxB infiltration to the distal end of the leaf was scored separately using 0 or 1 meaning the absence or presence of the symptom, respectively. This trait is noted as ‘ToxB_Spread’ in the data file.

Genotypic data: The GDP lines were genotyped using the Illumina iSelect 90K SNP array. Markers were filtered using previously published genetic maps, and marker sequences were aligned to the Svevo.v1 reference genome. SNP imputation was performed, and the redundant markers were pruned based on genome wide linkage disequilibrium, which resulted in a data set with no missing SNP calls. The hapmap information of 855 genotypes and 13,373 SNPs developed by M. Maccaferri is located on the GrainGenes website (https://wheat.pw.usda.gov/GG3/global_durum_genomic_resources). The haplotype map of the 855 genotypes was reduced to 510 genotypes that were selected for phenotypic tests. The SNP data set was selected according to the set filtering for minor allele frequency (MAF) > 5% that resulted in a marker set containing 12,222 SNPs.