Ag Data Commons
Browse
1/1
3 files

Data from: An in vitro analysis of how lactose modifies the gut microbiota structure and function of adults in a donor-independent manner

dataset
posted on 2023-12-18, 20:30 authored by Jenni Firrman, LinShu Liu, Karley K. Mahalak

The impact of lactose on the gut microbiota of healthy adults was examined, using a short-term, in vitro strategy where fecal samples harvested from 18 donors were cultured anaerobically with and without lactose. Donors represented 3 adult age groups. Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883645: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645), RT-qPCR of Bifidobacterium 16S rRNA genes, and short-chain fatty acid concentrations.

Resources in this dataset:

  • Resource Title: lactose sample attributes File Name: lactose_sample_metadata.csv

  • Resource Title: lactose SCFA, pH, and gas production data File Name: lactose_SCFA_data.csv

  • Resource Title: Total bacterial and Bifidobacterium genus qPCR File Name: lactose_qPCR_all_bacteria_and_bifido.csv

Funding

USDA-ARS: 8072-41000-108-000D

History

Data contact name

Narrowe, Adrienne

Data contact email

adrienne.narrowe@usda.gov

Publisher

Ag Data Commons

Temporal Extent Start Date

2021-06-01

Theme

  • Not specified

Geographic Coverage

{"type":"FeatureCollection","features":[{"geometry":{"type":"Point","coordinates":[-75.187092456102,40.078126249741]},"type":"Feature","properties":{}}]}

Geographic location - description

Ghent, Belgium and Philadelphia, PA, USA

ISO Topic Category

  • health

National Agricultural Library Thesaurus terms

lactose; intestinal microorganisms; sequence analysis; ribosomal RNA; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; Bifidobacterium; short chain fatty acids; fatty acid composition; nucleotide sequences

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 306

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Firrman, Jenni; Liu, LinShu; Mahalak, Karley K. (2023). Data from: An in vitro analysis of how lactose modifies the gut microbiota structure and function of adults in a donor-independent manner. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1529150

Usage metrics

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC