posted on 2023-12-18, 20:30authored byJenni Firrman, LinShu Liu, Karley K. Mahalak
<p>The impact of lactose on the gut microbiota of healthy adults was examined, using a short-term, in vitro strategy where fecal samples harvested from 18 donors were cultured anaerobically with and without lactose. Donors represented 3 adult age groups. Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883645: <a href="https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645">https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645</a>), RT-qPCR of Bifidobacterium 16S rRNA genes, and short-chain fatty acid concentrations. </p>
<p>Resources in this dataset:</p>
<ul>
<li>
<p>Resource Title: lactose sample attributes
File Name: lactose_sample_metadata.csv</p>
</li>
<li>
<p>Resource Title: lactose SCFA, pH, and gas production data
File Name: lactose_SCFA_data.csv</p>
</li>
<li>
<p>Resource Title: Total bacterial and Bifidobacterium genus qPCR
File Name: lactose_qPCR_all_bacteria_and_bifido.csv</p>
</li>
</ul>
Firrman, Jenni; Liu, LinShu; Mahalak, Karley K. (2023). Data from: An in vitro analysis of how lactose modifies the gut microbiota structure and function of adults in a donor-independent manner. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1529150