Data from: Aerosol transmission from infected swine to ferrets of an H3N2 virus collected from an agricultural fair and associated with human variant infections
A controlled influenza A virus transmission experiment was conducted in biosafety level 2 containment from January 23, 2018- February 13, 2018 assessing the respiratory droplet transmission efficiency of A(H3N2)/swine/Ohio/A01354299/2017 from experimentally inoculated pigs to naïve, contact ferrets. This experimental setting models the human-swine interface at agricultural fairs, where human infection with influenza viruses of swine origin has been well documented. Five pigs were housed in a standard enclosure and four ferrets were housed in isolators placed 7.5cm from the pig enclosure. Nasal swab samples from pigs were collected on 0, 1, 3, and 5 days post infection and nasal wash samples from ferrets were collected 0, 2, 4, 6, 8, and 15 days post contact. The whole genome sequences of 15 swine and 12 ferret virus isolates were obtained from respiratory samples cultured on Madin-Darby Canine Kidney cells. Sequencing was performed on an Illumina MiSeq platform and consensus sequences for each gene segment were generated using in-house software at the USDA-APHIS National Veterinary Services Laboratory.
Resources in this dataset:
Resource Title: Swine and Ferret Consensus Sequences.
File Name: Consensus_Sequences.txt
Resource Description: WGS consensus sequences of influenza virus from "Aerosol transmission from infected swine to ferrets of an H3N2 virus collected from an agricultural fair and associated with human variant infections".
Funding
USDA-ARS: 5030-32000-120-00-D
National Institute of Allergy and Infectious Diseases: HHSN272201400008C
National Institute of Allergy and Infectious Diseases: HHSN272201400006C
USDA-ARS: 0500-00093-001-00-D
Oak Ridge Institute for Science and Education: DE-AC05-06OR23100
History
Data contact name
Vincent, AmyData contact email
amy.vincent@usda.govPublisher
Ag Data CommonsIntended use
This data may be used to study the transmission and evolution of influenza A virus in swine and ferrets and additional data in support of publication.Temporal Extent Start Date
2018-01-23Temporal Extent End Date
2018-02-13Theme
- Not specified
ISO Topic Category
- biota
National Agricultural Library Thesaurus terms
genome; nose; swine; ferrets; aerosols; Influenza A virus; agricultural exhibitions; humans; airborne transmission; Orthomyxoviridae; virus transmission; nucleotide sequencesOMB Bureau Code
- 005:18 - Agricultural Research Service
OMB Program Code
- 005:040 - National Research
ARS National Program Number
- 103
Primary article PubAg Handle
Pending citation
- No
Public Access Level
- Public