Data from: A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat

Fusarium head blight (FHB), which is mainly caused by Fusarium graminearum, is a destructive wheat disease that threatens global wheat production. Fhb1, a quantitative trait locus discovered in Chinese germplasm, provides the most stable and the largest effect on FHB resistance in wheat. Here we show that TaHRC, a gene that encodes a putative histidine-rich calcium-binding protein, is the key determinant of Fhb1-mediated resistance to FHB. We demonstrate that TaHRC encodes a nuclear protein conferring FHB susceptibility and that a deletion spanning the start codon of this gene results in FHB resistance. Identical sequences of the TaHRC-R allele in diverse accessions indicate that Fhb1 had a single origin, and phylogenetic and haplotype analyses suggest that the TaHRC-R allele most likely originated from a line carrying the Dahongpao haplotype. This discovery opens a new avenue to improve FHB resistance in wheat, and possibly in other cereal crops, by manipulating TaHRC sequence through bioengineering approaches.

Supplementary Tables present

• Primers used for fine mapping, gene cloning, association mapping, diagnostic marker development and gene transformation
• A list of genes in the Fhb1 region based on the reference sequence of Chinese Spring and CM82036
• Differentially expressed genes between Fhb1 NILs that were identified by RNA-seq and mapped on chromosome 3B
• List of names, identification numbers (IDs), sources of wheat accessions and sequence polymorphisms in TaHRC
• Haplotype distribution of the three candidate genes across 1,632 wheat accessions from 73 countries worldwide
• Comparison of FHB resistance among haplotypes Hap_Ning, Hap_DHP and Hap_ND
  
  Resources in this dataset:
  
  

  • Resource Title: A Deletion Mutation in TaHRC Confers Fhb1 Resistance to Fusarium Head Blight in Wheat - Data Availability.

    File Name: Web Page, url: https://doi.org/10.1038/s41588-019-0425-8

    RNA-seq data are available from the NCBI Sequence Read Archive under accession PRJNA515933. Additional data generated or analyzed during this study are included in this article and its supplementary information files.