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Data From: Microplate bioassays to examine effects of grapevine-isolated stilbenoids on survival of root knot nematodes

posted on 2023-11-30, 11:02 authored by Christopher WallisChristopher Wallis

This dataset consists of a data file with raw data from a bioassay that assessed the survival of root knot nematode (J2 life-stage) in the presence of different stilbenoid compounds, as well as another file showing summary descriptive statistics and a summary graph. The experiment consisted of counting the survival of J2 stage root knot nematodes in microplate wells with water amended with 0, 1.25, 2.5, 5, or 10 ppm of the stilbenoid monomer piceid, the stilbenoid dimer epsilon-viniferin, the stilbenoid trimer miyabenol C, or a mix of stilbenoid tetramers vitisin B and hopeaphenol. Counts were made at the beginning of the experiment and 1, 3, or 5 days later. The percent of surviving nematodes at day 5 was used to observe if the stilbenoid compounds would have effects on the root knot nematodes.

Resources in this dataset:

  • Resource Title: RootKnotBioassayData.

    File Name: RootKnotBioassayData.xlsx

    Resource Description: Raw count data from an experiment assessing the mortality or survival of J2 root knot nematodes over time when exposed to piceid, epsilon-viniferin, miyabenol C, and vitisin B/hopeaphenol at 0, 1.25, 2.5, 5, or 10 ppm.

  • Resource Title: RootKnotBioassaySummary.

    File Name: RootKnotBioassaySummary.xlsx

    Resource Description: Descriptive statistics (means and deviations) and ANOVA statistics for survival percentage data of root knot nematodes exposed to four different stilbenoid compounds at different concentrations 1, 3, or 5 days after initial exposure. A graph of survival at day 5 is also provided.


Agricultural Research Service, 2034-22000-012-00D


Data contact name

Wallis, Christopher

Data contact email


Ag Data Commons

Intended use

Data is intended to support hypotheses and new research aimed at discovering potential mechanisms that resistant grapevine rootstocks might possess to limit infections by root knot nematodes. These data are to be considered still preliminary- follow-up studies will be warranted to provide further evidence that the assessed compounds are indeed associated with root knot nematode resistance.

Use limitations

Provided data consisted of a small study in terms of breadth and depth, mostly limited by the lack of access to the compounds utilized. Namely, the stilbenoid trimer miyabenol C and the tetramers vitisin B and hopeaphenol do not have commercially availability and had to be collected in a very time-consuming matter via liquid chromatography fraction collections. This study also did not utilize combinations of compounds, and only examined one life-stage of the nematodes (the free-living J2 stage). Lastly, this study was conducted in the rather artificial environment of amended water, which would be a very different environment than the soils that these nematodes normally dwell. All of these limitations should be considered in the interpretation of the provided results.

Temporal Extent Start Date


Temporal Extent End Date



  • Not specified

Geographic Coverage


Geographic location - description

Parlier, CA, USA

ISO Topic Category

  • farming

National Agricultural Library Thesaurus terms

bioassays; stilbenoids; root-knot nematodes; data collection; viniferins; Nematoda; Vitis

OMB Bureau Code

  • 005:18 - Agricultural Research Service

OMB Program Code

  • 005:040 - National Research

ARS National Program Number

  • 303

Pending citation

  • No

Public Access Level

  • Public

Preferred dataset citation

Wallis, Christopher M. (2022). Data From: Microplate bioassays to examine effects of grapevine-isolated stilbenoids on survival of root knot nematodes. Ag Data Commons.

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