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Coupling virus induced silencing with long and short term stress exposure in Fiskeby III soybeans

dataset
posted on 2024-01-27, 00:33 authored by USDA-ARS
Fiskeby III is a soybean variety uniquely resistant to a variety of abiotic stresses, including iron deficiency. To understand the Fiskeby III response to iron deficiency we have coupled bean pod mottle virus (BPMV) virus induced gene silencing (VIGS) with RNAseq analyses at two timepoints. Plants were infected with either an empty vector construct (VIGS_EV) or a construct to silence the candidate gene Glyma.05G001700 (VIGS_Glyma.05G001700). Plants were grown in hydroponics and moved to new hydroponic conditions, with half the plants moved to sufficient hydroponics and half moved to iron deficient (-Fe) conditions for either 1D or 7D of -Fe stress.

Funding

United Soybean Board, 2120-172-0145

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2022-09-09

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA878827

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