Coupling virus induced silencing with long and short term stress exposure in Fiskeby III soybeans

Fiskeby III is a soybean variety uniquely resistant to a variety of abiotic stresses, including iron deficiency. To understand the Fiskeby III response to iron deficiency we have coupled bean pod mottle virus (BPMV) virus induced gene silencing (VIGS) with RNAseq analyses at two timepoints. Plants were infected with either an empty vector construct (VIGS_EV) or a construct to silence the candidate gene Glyma.05G001700 (VIGS_Glyma.05G001700). Plants were grown in hydroponics and moved to new hydroponic conditions, with half the plants moved to sufficient hydroponics and half moved to iron deficient (-Fe) conditions for either 1D or 7D of -Fe stress.