posted on 2024-02-13, 13:45authored byTaein Lee, Sook Jung, Ksenija Gasic, Todd Campbell, Jing Yu, Jodi Humann, Heidi Hough, Dorrie Main
Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:
oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,
PCR product size,
positional constraints within the source (template) sequence, and
possibilities for ectopic priming (amplifying the wrong sequence)
many other constraints.
All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.
Resources in this dataset:
Resource Title: Website Pointer for CottonGen Primer3.