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Characterization of NgAgo cleavage sites

dataset
posted on 2024-11-23, 22:12 authored by Purdue University
NgAgo and mutants were incubated with plasmid DNA and 5'P guide sequences targeting the plasmid. Nicked/cleaved products were amplified via primer extension, which terminates at the nick site, before being used to generate dsDNA that was ligated into pGEM-T-Easy. The library of ligated products were then sequenced. Raw reads from products generated via primers hybridizing on the 5' and 3' end of the target are deposited here.

Funding

USDA-NIFA: Hatch Multistate Project S1041

RGSRT: Award #41000622

PRF: #60000025 & #60000029

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2021-07-20

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA748512

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA748512 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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