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Cattle antibody repertoires before and after vaccination against bovine respiratory disease

dataset
posted on 2024-01-26, 23:51 authored by USDA
Purebred American Angus calves (n = 204) were vaccinated with a modified live vaccine (Bovi-Shield Gold 5; Zoetis Inc., Parsippany N.J.) containing antigens of 4 viruses associated with bovine respiratory disease as described (Kramer et al., 2017). A second booster vaccination was applied three weeks later. Bovine whole blood was collected and stored in Tempus TM blood RNA tubes (Applied Biosystems, Foster City, CA). Collections occurred at four-time points; three weeks prior to vaccination (Pre), at vaccination (Day 0), three weeks post-vaccination at booster vaccination (3 week), and 3 weeks after booster vaccination (6 week). The RNA was converted to cDNA using primers designed for capturing IgG and sequenced using Illumina MiSeq.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2020-02-21

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA607961

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