Broiler cardiac response to heat stress

Two broiler lines with different growth rate were divided to two groups. One was subjected to heat stress at 35 Celsius degree from 21 days to 42 days posthatch, but the other was kept in thermoneutral condition. Gene expression in left ventricle at 42 days posthatch was measured by RNA-seq and compared between different treatments and chicken lines. The purpose of this study is to investigate cardiac gene expression change triggered by heat stress and to find possible pathways related to differential resistance to heat stress in two broiler lines.