Ag Data Commons
Browse

File(s) stored somewhere else

Please note: Linked content is NOT stored on Ag Data Commons and we can't guarantee its availability, quality, security or accept any liability.

Auxenochlorella protothecoides Transcriptome or Gene expression

dataset
posted on 2024-01-26, 22:53 authored by USDA ARS
These data contain transcriptome profiles of the alga C. protothecoides under two different growth regimes and at three different stages of cell growth. Cultivation of the organism and total RNA extraction was conducted at the USDA-ARS Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center in Hilo, Hawaii during September 2013; subsequently, samples were submitted to the Yale Center for Genomic Analysis from Illumina Hi-Seq library preparation and sequencing. Synchronized cultures were grown in either photoautotrophic medium (Bristol's salts plus 0.1% (w/v) proteose peptone) or papaya-based heterotrophic medium (P1, Bristol's salts plus 12.5% (v/v) Rainbow papaya juice). Samples containing 1.0E+08 cells were harvested from each of three biological replicate cultures grown in both medium compositions during the logarithmic growth phase. Additionally, samples were harvested from the heterotrophic cultures once the cell density had nearly saturated (the transitional point between growth and oil biosynthesis), and again during peak oil biosynthesis.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2015-07-07

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA289168

Usage metrics

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC