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Arabidopsis thaliana Raw sequence reads

dataset
posted on 2025-04-02, 02:50 authored by Duke University
Our genetic and biochemical analyses indicated that ARF6 and ARF8 are downstream of SPY in regulating parthenocarpy. To identify ARF6, ARF8 and SPY responsive genes that are involved in fruit set and growth, transcriptome analysis was performed by RNA-seq using the following samples, unpollinated pistils at 2 days before anthesis, from stage 10 flowers of arf6-2, arf8-3, spy-3 and WT; and 0 DAA WT pistils from stage 14 flowers that were already self-pollinated. Two biological repeats were included in each set of samples. Total RNAs were purified from unpollinated pistils from stage 10 flowers of arf6-2, arf8-3, spy-3 and WT, and pollinated pistils from WT stage 14 flowers (0 DAA). RNA-Seq cDNA libraries, two biological repeats, were prepared with the Quant Seq mRNA-Seq library prep kit FWD for Illumina.

Funding

NIH: GM150029

NIGMS NIH HHS: R01 GM100051

USDA: 2023-67013-39532

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2024-04-02

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA1095421

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA1095421 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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