Analysis of Fusarium verticillioides gene expression in response to nitric oxide (NO)
dataset
posted on 2024-09-29, 05:51authored byToxicology & Mycotoxin Research Unit, USDA, ARS, USNPRC, R.B. Russell Research Center
Investigation of whole transcriptional changes in F. verticillioides wild-type (WT) strain FRC M-3125 and gene deletion strain ΔFVEG_10494-6.4 when treated with and without 1.5 mM diethylenetriamine (DETA) NONOate for 30 min, with three biological replicates each. DETA NONOate is a NO-donor molecule. Overall design: Strains were grown for three days in potato dextrose broth (PDB) buffered with 0.1 M sodium phosphate. Prior to harvesting samples, the NO treatments received a 30 min exposure to 1.5 mM DETA NONOate dissolved in 0.01 M NaOH.
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