Ag Data Commons
Browse

File(s) stored somewhere else

Please note: Linked content is NOT stored on Ag Data Commons and we can't guarantee its availability, quality, security or accept any liability.

Analysis of Fusarium verticillioides gene expression in response to nitric oxide

dataset
posted on 2024-01-26, 22:47 authored by Toxicology & Mycotoxin Research Unit, USDA, ARS, USNPRC, R.B. Russell Research Center
Investigation of whole genome gene expression level changes in F. verticillioides M3125 when exposed to 1.5 mM DETA NONOate for 30 minutes. Assessed in reference to unexposed M3125. DETA NONOate is a nitric oxide (NO) donor with a half-life of approximately 56 hours at 27°C, pH 7.4, to liberate 2 moles of NO per mole of parent compound. Overall design: A four chip study using total RNA recovered from two separate control cultures of F. verticillioides M3125 in PDB buffered with 0.1 M sodium phosphate and two separate experimental cultures of M3125 grown in the same buffered PDB plus the added treatment of 1.5 mM DETA NONOate. The cultures were incubated for 30 minutes and then harvested by vacuum filtration and frozen in liquid nitrogen. The chips are a F. verticillioides custom 14K array based on platform GPL14669.

History

Data contact name

BioProject Curation Staff

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2014-04-15

Theme

  • Non-geospatial

ISO Topic Category

  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

Pending citation

  • No

Public Access Level

  • Public

Accession Number

PRJNA244692

Usage metrics

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC