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Analysis of Fusarium verticillioides gene expression in response to lactam and lactone compounds

posted on 2024-06-11, 05:53 authored by Toxicology & Mycotoxin Research Unit, USDA, ARS, USNPRC, R.B. Russell Research Center
Investigation of whole transcriptional changes in F. verticillioides FRC M-3125 when exposed to 5 μg/ml pyrrocidine A (PA), 20 μg/ml pyrrocidine B (PB), 50 μg/ml 2-benzoxazolinone (BOA), 50 μg/ml 2-oxindole (OXD), 50 μg/ml 2-coumaranone (CMN), or 50 μg/ml chlorzoxazone (CZX). Cultures were harvested one hour after exposure. Assessed in reference to control cultures of M-3125 exposed to DMSO (0.5% final concentration) since all the above compounds were dissolved in DMSO. Overall design: Two milliliters of PDB cultures in sterile snap-cap tubes, with caps loose, were seeded with 10000 FRC M-3125 wild-type F. verticillioides conidia and grown at 27 °C, 250 rpm for 47 hours, at which time the respective cultures were treated [10 μL DMSO containing 100 μg of either BOA, OXD, CMN, or CZX (final concentrations of 50 μg/mL); or 10 μg of PA (5 μg/m final); or 40 μg of PB (20 μg/m final)]. Ten microliters of DMSO was added to control samples. All culture tubes were incubated for a final one-hour induction. After induction, total RNA was extracted.


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National Center for Biotechnology Information

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  • Non-geospatial

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  • biota

National Agricultural Library Thesaurus terms

transcriptome; gene expression

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  • Public

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It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA478232 in the NCBI BioProject database ("

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