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An efficient multiplex approach to CRISPR/Cas9 gene editing in Citrus

Name: An efficient multiplex approach to CRISPR/Cas9 gene editing in Citrus
Published: 2024-09-29T07:17:08+00:00
License: https://rightsstatements.org/vocab/UND/1.0/
Keywords: raw sequence reads

dataset

posted on 2024-09-29, 07:17 authored by Yale University

This study evaluates different promoters, Cas9 variants, and strategies to express multiplexed sgRNAs for the purpose to of defining improved vectors that can be used to efficiently edit four genes at once in Citrus. We show that the ES8Z promoter from Arabidopsis is an effective promoter in Citrus, and can be used to drive tRNA arrays for multiplex gene editing. In addition, define a set of components that are highly effective in multiplex gene editing.

Funding

USDA: 2023-70029-41277

History

Data contact name

BioProject Curation Staff

Data contact email

bioprojecthelp@ncbi.nlm.nih.gov

Publisher

National Center for Biotechnology Information

Temporal Extent Start Date

2024-03-21

Theme

Non-geospatial

ISO Topic Category

biota

National Agricultural Library Thesaurus terms

sequence analysis

Pending citation

Public Access Level

Public

Accession Number

PRJNA1090434

Preferred dataset citation

It is recommended to cite the accession numbers that are assigned to data submissions, e.g. the GenBank, WGS or SRA accession numbers. If individual BioProjects need to be referenced, state that "The data have been deposited with links to BioProject accession number PRJNA1090434 in the NCBI BioProject database (https://www.ncbi.nlm.nih.gov/bioproject/)."

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Keywords

raw sequence reads

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